Analysis of Epithelial Cell Surface Polarity Development with Monoclonal Antibodies
The cells of transporting epithelia are organized into either sheets or tubules so that they provide a barrier between two compartments, the mucosal and serosal, which are essential for the proper physiological functioning of a variety of organs and tissues. The plasma membrane of epithelial cells is divided into two unique domains: the apical membrane, which borders the mucosal or luminal side, and the basolateral membrane, which contacts the basal lamina on the serosal side of the epithelium (Berridge and Oschman, 1972). At the boundary between the apical and basolateral domains is a differentiated region of the plasma membrane termed the junctional complex (Farquhar and Palade, 1963), and one of these membrane specializations, the tight junction, seals the lateral space, preventing the transepithelial movement of ions and larger molecules (Farquhar and Palade, 1963; Staehelin, 1974).
KeywordsTight Junction MDCK Cell Basolateral Membrane Apical Plasma Membrane Distal Convoluted Tubule
Unable to display preview. Download preview PDF.
- Berridge, M. J., and Oschman, J. L., 1972, Transporting Epithelia, Academic Press, New York.Google Scholar
- Blobel, G., Walter, P., Chung, C. N., Goldman, B. M., Erickson, A. H., and Lingappa, V. R., 1979, Translocation of proteins across membranes: The signal hypothesis and beyond, Symp. Soc. Exp, Biol. 33:9–36.Google Scholar
- Matlin, K. S., Bainton, D. F., Personen, M., Louvard, D., Gentry, N., and Simons, K., 1983, Transepithelial transport of a viral membrane glycoprotein implanted into the apical plasma membrane of Madin-Darby canine kidney cells. I. Morphological evidence, J. Cell Biol. 97:627–637.PubMedCrossRefGoogle Scholar
- Reggio, H., Courdrier, E., and Louvard, D., 1982, Surface and cytoplasmic domains in polarized epithelial cells, in: Progress in Clinical and Biological Research Vol. 91 (J. F. Hoffman, G. H. Giebisch, L. Doris, eds.) Alan R. Liss, New York, pp. 89–105.Google Scholar
- Rodriguez-Boulan, E., 1983, Membrane biogenesis, enveloped RNA viruses, and epithelial polarity, in:Modern Cell Biology, Vol. 1 (B. Satir, ed.) Alan R. Liss, New York, pp. 119–170.Google Scholar
- Semenza, G., 1979, Small intestinal disaccharidases: Their properties and role as sugar translocators across natural and artificial membranes, in: The Enzymes of Biological Membranes, Vol. 3 (A. Martonosi, ed.), Plenum Press, New York, pp. 349–382.Google Scholar
- Van Meer, G., and Simons, K., 1982, Viruses budding from either the apical or the baso-lateral plasma membrane domain of MDCK cell have unique phospholipid compositions, EMBOJ. 1:847–852.Google Scholar