Cell—Cell Interactions in the Development of Dictyostelium

  • Donna Fontana
  • Tit-Yee Wong
  • Anne Theibert
  • Peter Devreotes
Part of the Developmental Biology book series (DEBO, volume 3)


Dictyostelium discoideum is a cellular slime mold that, because of its intricate life cycle (Fig. 1), has attracted the attention of developmental biologists. With an adequate supply of food, amoebae of D. discoideum will grow and divide as individual cells. When the food supply is depleted (in the laboratory, this is accomplished by removing a nutrient broth or bacteria), the amoebae cease growing and enter into a developmental program. There is a period of protein synthesis and, if the amoebae are on a solid surface, this is followed by the cAMP-mediated aggregation of 105–106 amoebae into a single mound. This mound becomes encased in slime (hence the name slime mold) and sends up a fingerlike projection that eventually includes most of the amoebae. This projec tion falls over onto the agar surface and begins to crawl; this is the pseudoplasmodial or slug stage. During this migration, the amoebae differentiate into either prestalk or prespore cells. After a period of time determined by genetic and environmental factors, the prestalk cells that form the anterior section of the slug cease moving, while the prespore cells located in the posterior section of the slug continue migrating until another moundlike structure is formed. The prestalk cells then begin depositing cellulosic walls and push down through the prespore cells to the agar surface, forming a stalk. When the cells are in their proper position and differentiation is complete, the stalk cells die, leaving their walls to support a ball-like structure that contains the mature spores.


Adenylate Cyclase Dictyostelium Discoideum Chemotactic Response cAMP Analogue Photoaffinity Label 


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  1. Bonner, J. T., 1982, Comparative biology of cellular slime molds, in: The Development of Dicyostelium discoideum (W. Loomis, Jr., ed.), PP- 1–33, Academic Press, New York.Google Scholar
  2. Brenner, M., and Thorns, S. D., 1984, Caffeine blocks activation of cyclic AMP synthesis in DicyosteJium discoideum, Dev. Biol. 101:136–146.PubMedCrossRefGoogle Scholar
  3. Condeelis, J., 1979, Isolation of Concanavalin A caps during various stages of formation and their association with actin and myosin, J. Cell Biol. 80:751–758.PubMedCrossRefGoogle Scholar
  4. Devreotes, P. N., 1982, Chemotaxis, in: The Development of Dictyostelium discoideum (W. Loomis, Jr., ed.), pp. 117–168, Academic Press, New York.Google Scholar
  5. Devreotes, P. N., and Steck, T. L., 1979, Cyclic 3’,5’-AMP relay in Dictyostelium discoideum. II. Requirements for initiation and termination of the response, J. Cell Biol. 80:300–309.PubMedCrossRefGoogle Scholar
  6. Devreotes, P. N., Derstine, P. L., and Steck, T. L., 1979, Cyclic AMP relay in Dictyostelium discoideum. I. A technique to monitor responses to control stimuli, J. Cell Biol. 80:291–299.PubMedCrossRefGoogle Scholar
  7. Dinauer, M., MacKay, S., and Devreotes, P., 1980a, Cyclic 3’,5’-AMP relay in Dictyostelium discoideum. III. The relationship of cAMP synthesis and secretion during the cAMP signaling response, J. Cell Biol. 86:537–544.PubMedCrossRefGoogle Scholar
  8. Dinauer, M., Steck, T., and Devreotes, P., 1980b, Cyclic 3’,5’-AMP relay in Dictyostelium discoideum. IV. Recovery of the cAMP signaling response after adaptation to cAMP, J. Cell Biol. 86:545–553.PubMedCrossRefGoogle Scholar
  9. Fontana, D. R., and Devreotes, P. N., 1984, cAMP-stimulated adenylate cyclase activation in Dicyostelium discoideum is inhibited by agents acting at the cell surface, Dev. Biol. 106:76–82.PubMedCrossRefGoogle Scholar
  10. Gillette, M. U., Dengler, R. E., and Filosa, M. F., 1974, The localization and fate of Concanavalin A in amoebae of the cellular slime mold, Dictyostelium discoideum, J. Exp. Zool. 190:243–248.PubMedCrossRefGoogle Scholar
  11. Gunther, G. R., Wang, J. L., Yahara, I., Cunningham, B. A., and Edelman, G. M., 1973, Concanavalin A derivatives with altered biological activities, Proc. Natl. Acad. Sci. USA 70:1012–1016.PubMedCrossRefGoogle Scholar
  12. Hellio, R., and Ryter, A., 1980, Relationships between anionic sites and lectin receptors in the plasma membrane of Dictyostelium discoideum and their role in phagocytosis, J. Cell Sci. 41:89–104.PubMedGoogle Scholar
  13. Juliani, M., and Klein, C.,1981, Photoaffinity labeling of the cell surface adenosine 3\5’-monohosphate receptor of Dictyostelium discoideum and its modification in down-regulated cells, J. Biol. Chem. 256:613–619.PubMedGoogle Scholar
  14. Konijn, T., 1970, Microbiological assay of cyclic 3’,5’-AMP, Experientia 26:367–369.CrossRefGoogle Scholar
  15. Leichtling, B. H., Coffman, D. S., Yaeger, E. S., Rickenberg, H. U., Al-Jumaliy, W., and Haley, B. E., 1981, Occurrence of the adenylate cyclase “G protein” in membranes of Dictyostelium discoideum, Biochem. Biophys. Bes. Commun. 102:1187–1195.CrossRefGoogle Scholar
  16. Luna, E. J., Fowler, V. M., Swanson, J., Branton, D., and Taylor, D. L., 1981, A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin binding activity, J. Cell Biol. 88:396–409.PubMedCrossRefGoogle Scholar
  17. Pan, P., Hass, E. M., and Bonner, J. T., 1972, Folic acid as second chemotactic substance in the cellular slime molds, Nature New Biol. 237:181–191.PubMedGoogle Scholar
  18. Roos, W., and Gerisch, G., 1976, Receptor mediated adenylate cyclase activation in Dictyostelium, FEBS Lett. 68:170–172.PubMedCrossRefGoogle Scholar
  19. Ross, E. M., and Gilman, A. G., 1980, Biochemical properties of hormone-sensitive adenylate cyclase, Annu. Rev. Biochem. 49:533–564.PubMedCrossRefGoogle Scholar
  20. Spudich, J. A., and Spudich, A., 1982, Cell motility, in: The Development of Dictyostelium discoideum (W. Loomis, Jr., ed.), pp. 169–194, Academic Press, New York.Google Scholar
  21. Theibert, A., and Devreotes, P., 1983, Cyclic 3\5’-AMP relay in Dictyostelium discoideum Adaptation is independent of activation of adenylate cyclase, J. Cell Biol. 97:173–177.PubMedCrossRefGoogle Scholar
  22. Tomchik, K. J., and Devreotes, P. N., 1981, cAMP waves in Dictyostelium discoideum Demonstration by a novel isotope dilution fluorography technique, Science 212:443–446.PubMedCrossRefGoogle Scholar
  23. van Haastert, P., 1983a, Sensory adaptation of Dictyostelium discoideum cells to chemotactic signals, J. Cell Biol. 96:1559–1565.PubMedCrossRefGoogle Scholar
  24. van Haastert, P. 1983b, Binding of cAMP and adenosine derivatives to Dictyostelium discoideum cells. Relationships of binding, chemotactic, and antagonistic activities, J. Biol. Chem. 258:9643–9648.PubMedGoogle Scholar
  25. van Haastert, P., and Kien, E., 1983, Binding of cAMP derivatives to DictyosteJium discoideum cells. Activation mechanism of the cell surface cAMP receptor, J. Biol. Chem. 258:9636–9642.PubMedGoogle Scholar
  26. Wallace, L., and Frazier, W., 1979, Photoaffinity labeling of cyclic-AMP and AMP-binding proteins of differentiating Dictyostelium discoideum cells, Proc. Nstl. Acad. Sci. USA 76:4250–4254.CrossRefGoogle Scholar
  27. Wang, J. L., Gunther, G. R., and Edelman, G. M., 1976, Properties of dimeric Con A derivatives, in: Concanavalin A as a Tool (H. Bittiger and H. P. Schnebli, eds.), pp. 581–595, Wiley, London.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Donna Fontana
    • 1
  • Tit-Yee Wong
    • 2
  • Anne Theibert
    • 1
  • Peter Devreotes
    • 1
  1. 1.Department of Biological ChemistryJohns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Department of BiologyJohns Hopkins UniversityBaltimoreUSA

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