Skip to main content
SpringerLink
Log in

Diffraction Studies on Crystals of Ribosomal Particles

  • Chapter
Structure and Function of the Genetic Apparatus

Part of the book series: NATO ASI Series ((NSSA,volume 98))

Abstract

One of the most fascinating biochemical processes is the biosynthesis of protein molecules. This intricate and accurate process takes place in all organisms on their ribosomes in a similar manner. It involves specific interactions of the ribosomes with mRNAs, aminoacyl-tRNAs and a number of proteins such as initiation, elongation and termination factors. During this process the ribosomes dissociate into their two subunits which reassociate after the initiation process. Each of these subunits is a structurally independent distinct assembly of many proteins and several RNA chains.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 16.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. G. Chambliss, G. R. Craven, J. Davies, K. Davis, L. Kahan, and M. Nomura (eds), “Ribosomes: Structure, Function and Genetics,” University Park Press, Baltimore (1980).

    Google Scholar 

  2. H. G. Wittmann, Components of bacterial ribosomes, Annual Rev. Biochem. 51:155–183 (1982).

    Article  CAS  Google Scholar 

  3. A. Liljas, Structural studies of ribosomes, Progr. Biophys. Mol. Biol. 40:161–228 (1982).

    Article  CAS  Google Scholar 

  4. H. G. Wittmann, Architecture of prokaryotic ribosomes, Ann. Rev. Biochem. 52:35–65 (1983).

    Article  PubMed  CAS  Google Scholar 

  5. J. Lake and H. S. Slayter, Three-dimensional structure of chromatoid body helix of Entamoeba invadens, J. Mol. Biol. 66:271–282 (1972).

    Article  PubMed  CAS  Google Scholar 

  6. W. Kühlbrandt and P. N. T. Unwin, Distribution of RNA and protein in crystalline eukaryotic ribosomes, J. Mol. Biol. 156:431–448 (1982).

    Article  PubMed  Google Scholar 

  7. R. A. Milligan and P. N. T. Unwin, In vitro crystallization of ribosomes from chick embryos, J. Cell. Biol. 95:648–653 (1982).

    Article  PubMed  CAS  Google Scholar 

  8. L. O’Brien, K. Shelley, J. Towfighi, and A. McPherson, Crystalline ribosomes are present in brains from senile humans, Proc. Natl. Acad, Sci. USA 77:2260–2264 (1980).

    Article  Google Scholar 

  9. M. W. Clark, M. Hammons, J. A. Langer, and J. A. Lake, Helical arrays of Escherichia coli small ribosomal subunits produced in vitro, J. Mol. Biol. 135:507–512 (1979).

    Article  PubMed  CAS  Google Scholar 

  10. M. W. Clark, K. Leonard, and J. A. Lake, Ribosomal crystalline arrays of large subunits from Escherichia coli, Science 216:999–1001 (1982).

    Article  PubMed  CAS  Google Scholar 

  11. T. Arad, K. Leonard, H. G. Wittmann, and A. Yonath, Two-dimensional crystalline sheets of Bacillus stearothermophilus 50S ribosomal particles, The EMBO J. 3:127–131 (1984).

    CAS  Google Scholar 

  12. A. Yonath, J. Müssig, and H. G. Wittmann, Parameters for crystal growth of ribosomal subunits, J. Cell. Biochem. 19:145–155 (1982).

    Article  PubMed  CAS  Google Scholar 

  13. A. Yonath, J. Müssig, B. Tesche, S. Lorenz, V. A. Erdmann, and H. G. Wittmann, Crystallization of the large ribosomal subunits from Bacillus stearothermophilus, Biochem. Internat. 1:428–453 (1980).

    CAS  Google Scholar 

  14. A. Yonath, B. Tesche, S. Lorenz, J. Mussig, V. A. Erdmann, and H. G. Wittmann, Several crystal forms of the Bacillus stearothermophilus 50S ribosomal particles, FEBS Lett. 154:15–20 (1983).

    Article  PubMed  CAS  Google Scholar 

  15. A. Yonath, J. Piefke, J. Mussig, H. S. Gewitz, and H. G. Wittmann, A compact three-dimensional crystal form of the large ribosomal subunits from Bacillus stearothermophilus, FEBS Lett. 163:69–72 (1983).

    Article  PubMed  CAS  Google Scholar 

  16. H. G. Wittmann, J. Mussig, J. Piefke, H. S. Gewitz, H. J. Rheinberger and A. Yonath, Crystallization of Escherichia coli ribosomes, FEBS Lett. 146:217–220 (1982).

    Article  PubMed  CAS  Google Scholar 

  17. K. R. Leonard, T. Arad, B. Tesche, V. A. Erdmann, H. G. Wittmann, and A. Yonath, Crystallization, electron microscopy and three-dimensional reconstruction studies of ribosomal subunits, 10th Internatl. Congr. Electron Microscopy, Hamburg, Vol. 3:9–15 (1984).

    Google Scholar 

  18. A. Yonath, H. D. Bartunik, K. S. Bartels, and H. G. Wittmann, Some X-ray diffraction patterns from single crystals of the large ribosomal subunit from Bacillus stearothermophilus, J. Mol. Biol. 177:201–206 (1984).

    Article  PubMed  CAS  Google Scholar 

  19. G. Zubay and M. H. F. Wilkins, X-ray diffraction studies on the structure of ribosomes from Escherichia coli, J. Mol. Biol. 2:105–112 (1960).

    Article  CAS  Google Scholar 

  20. A. Klug, K. C. Holmes, and J. T. Finch, X-ray diffraction studies on ribosomes from various sources, J. Mol. Biol. 3:87–100 (1961).

    Article  PubMed  CAS  Google Scholar 

  21. R. Langridge and K. C. Holmes, X-ray diffraction studies of concentrated gels of ribosomes from Escherichia coli, J. Mol. Biol. 5:611–617 (1962).

    Article  PubMed  CAS  Google Scholar 

  22. A. Yonath, G. Khavitch, B. Tesche, J. Müssig, S. Lorenz, V. A. Erdmann, and H. G. Wittmann, The nucleation of crystals of the large ribosomal subunits from Bacillus stearothermophilus, Biochem. Internat. 5:629–636 (1982).

    CAS  Google Scholar 

  23. T. J. Richmond, J. T. Finch, and A. Klug, Studies on nucleosome structure, Cold Spring Harbor Symp. 47:493–501 (1982).

    Article  CAS  Google Scholar 

  24. G. A. Bentley, J. T. Finch, and A. Lewit-Bentley, Neutron diffraction studies on crystals of nucleosome cores using contrast variation, J. Mol. Biol. 145:771–784 (1981).

    Article  PubMed  CAS  Google Scholar 

  25. A. Shevack, H. S. Gewitz, B. Hennemann, A. Yonath and H. G. Wittmann, Characterization and crystallization of ribosomal particles from Halobacterium marismortui, FEBS Lett. 184:68–71 (1985).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Molekulare Genetik, D-1000, Berlin 33, Germany

    H. G. Wittmann

  2. Department of Structural Chemistry, Weizmann Institute, Rehovot, Israel

    A. Yonath

Authors
  1. H. G. Wittmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Yonath
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Temple University, Philadelphia, Pennsylvania, USA

    Claudio Nicolini

  2. Johns Hopkins University, Baltimore, Maryland, USA

    Paul O. P. Ts’o

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Plenum Press, New York

About this chapter

Cite this chapter

Wittmann, H.G., Yonath, A. (1985). Diffraction Studies on Crystals of Ribosomal Particles. In: Nicolini, C., Ts’o, P.O.P. (eds) Structure and Function of the Genetic Apparatus. NATO ASI Series, vol 98. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5024-8_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-5024-8_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5026-2

  • Online ISBN: 978-1-4684-5024-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation