Liposome-Entrapped Enzyme Mediated Immunoassays
Liposomes, first described by Bangham, et al.(1965), are membrane vesicles that can form spontaneously by suspending a variety of lipids in water. These vesicles can be prepared as large multilamellar structures containing many internal aqueous compartments or as smaller unilamellar structures with only one internal compartment. If prepared in the presence of marker molecules, such as soluble enzymes, ions or flurophores, the markers are entrapped within the aqueous spaces surrounded by bilayer lipid membranes. In addition, liposome membranes can be labeled with either antigens or antibodies, and they can be lysed by a variety of agents.
KeywordsBilayer Lipid Membrane Liposome Preparation Dose Curve Free Antigen Entrap Enzyme
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