Nonseparation Enzyme Immunoassays for Macromolecules
Two classes of homogeneous enzyme immunoassays for macromolecules have been developed at the Syva Research Institute. The first class uses β-galactosidase together with a macromolecular substrate (Gibbons et al., 1980). The second is based on the phenomenon of “enzyme channeling” (Litman et al., 1980) involving two or more enzymes. This chapter will discuss the principles, characteristics, performance and clinical utility of both types of assay.
KeywordsVortex Hydrolysis Albumin Anemia Fructose
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