Enzyme Modulator as Label in Separation-Free Immunoassays: Enzyme Modulator Mediated Immunoassay (EMMIA)

  • T. T. Ngo


Enzyme modulator mediated immunoassay (EMMIA) is a separation-free enzyme amplified immunoassay. The assay uses an enzyme modulator (M) as the tag to label a ligand analyte (L). Such stable covalent enzyme modulator-ligand conjugates (M-L) is capable of modulating the activity of an indicator enzyme by either causing a significant inhibition of the enzyme activity or a dramatic activation.


Dihydrofolate Reductase Pyruvate Carboxylase Enzyme Modulator Transition State Analog Isobutyl Chloroformate 


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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • T. T. Ngo
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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