Immunoperoxidase Techniques Using the Avidin-Biotin System
Although immunofluorescence techniques were first develpoed in the early 1960s, they did not gain widespread use and acceptance until the following decade, when they became invaluable tools for the resolution of a number of problems in experimental immunology. Since then, the method has been used clinically for the identification of surface and intracellular lymphocyte markers and immune complexes of glomerular diseases. Within the past 15 years, an alternative method using enzyme-labeled antibodies has been developed which is rapidly replacing immunofluorescence because of its simplicity and usefulness in formalin-fixed paraffin embedded tissue sections. Although many enzymes have been sucessfully conjugated to various antibodies, most recent studies have employed horseradish peroxidase. The amount of antigens in tissue sections is generally determined semi-quantitatively by examining the staining intensity using serial diluted antibodies (Hsu et al., 1981a,b). More recently, the immunoperoxidase techniques have been adapted to other solid phase systems, such as enzyme-linked immunoassys or western blots of antigens (Azen and Yu, 1984; Madri and Barwick, 1983; Rosenkoetter et al., 1984).
KeywordsClin Pathol Immunoperoxidase Technique Peanut Agglutinin Solid Phase System Avidin Molecule
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