Development of Immunochemical Enzyme Assays for Cardiac Isoenzymes

  • Richard Wicks
  • Magdalena Usategui-Gomez


The term isoenzyme is applied to proteins that catalyze the same chemical reaction, yet are structurally different. This heterogeneity of enzymes has been known for several decades (Vessell, 1958; Cahn, 1962; Markert, 1963; Nisselbaum, 1963). In the last few years, isoenzyme determinations have become very important in the clinical laboratory because they have proven to be powerful diagnostic tools since the various tissues and organs of man exhibit characteristic isoenzyme profiles. When an infusion of particular isoenzymes into the circulation occurs due to damage to a specific tissue or organ, an isoenzyme determination can often pinpoint the site of injury. After a myocardial infarction (MI), isoenzymes of lactate dehydrogenase (LD) and creatine kinase (CK) are released from the myocardial tissue into the circulation. We have utilized the different immunochemical properties of CK and LD isoenzymes to develop simple, rapid and precise immunoassays for determining the specific cardiac isoenzymes of both enzymes.


Acute Myocardial Infarction Creatine Kinase Immunochemical Method Mitochondrial Creatine Kinase Total Creatine Kinase 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Richard Wicks
    • 1
  • Magdalena Usategui-Gomez
    • 1
  1. 1.Department of Diagnostic ResearchHoffmann-La Roche Inc.NutleyUSA

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