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Development of Immunochemical Enzyme Assays for Cardiac Isoenzymes

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Enzyme-Mediated Immunoassay

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Abstract

The term isoenzyme is applied to proteins that catalyze the same chemical reaction, yet are structurally different. This heterogeneity of enzymes has been known for several decades (Vessell, 1958; Cahn, 1962; Markert, 1963; Nisselbaum, 1963). In the last few years, isoenzyme determinations have become very important in the clinical laboratory because they have proven to be powerful diagnostic tools since the various tissues and organs of man exhibit characteristic isoenzyme profiles. When an infusion of particular isoenzymes into the circulation occurs due to damage to a specific tissue or organ, an isoenzyme determination can often pinpoint the site of injury. After a myocardial infarction (MI), isoenzymes of lactate dehydrogenase (LD) and creatine kinase (CK) are released from the myocardial tissue into the circulation. We have utilized the different immunochemical properties of CK and LD isoenzymes to develop simple, rapid and precise immunoassays for determining the specific cardiac isoenzymes of both enzymes.

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References

  • Ali, M., Braun, E.V., Laraia, S., Olusegun, F., Nalebuff, D., and Palladino, P.H. (1981). Immunochemical assay for myocardial infarction. Amer. J. Clin. Path. 76:426–429.

    CAS  Google Scholar 

  • Ali, M., Laraia, S., Angeli, R., et al. (1982). Immuno chemical CK-MB assay for myocardial infarction. Amer. J. Clin. Pathol. 77:573–579.

    CAS  Google Scholar 

  • Bark, C.J. (1980). Mitochondrial creatine kinase. J. Amer. Med. Assoc. 243:2058–2060.

    Article  CAS  Google Scholar 

  • Bayer, P.M., Wider, G., Unger, W., et al. (1982). Atypische kreatinkinase isoenzyme. Klin. Wochenschr. 60:365–369.

    Article  PubMed  CAS  Google Scholar 

  • Bohner, J., Stein, W., Steinhart, R., et al. (1982). Macro creatine kinases: Results of isoenzyme electrophoresis and differentiation of the immunoglobulin-bound type by radio assay. Clin. Chem. 28:618–623.

    PubMed  CAS  Google Scholar 

  • Boll, M., Backs, M., and Pfleiderer, G. (1974). Quantitative Immunologische bestimming von multipler formen der lactat-dehydrogenase in tierischem un humanen gewebe. Z. Physiol. Chem. 355:811–818.

    Article  CAS  Google Scholar 

  • Bruns, D.E., Emerson, J.C., Intemann, S., Bertholf, R., Hill, K.E., and Savory, J. (1981). Lactate dehydrogenase isoenzyme-1: Changes during the first day after acute myocardial infarction. Clin. Chem. 27:1821–1823.

    PubMed  CAS  Google Scholar 

  • Bruns, D., Chitwood, J., Koller, K., et al. (1983). Creatine kinase MB activity: Clinical and laboratory studies of specific immunochemical technique with optimized enzymatic assay. Ann. Lab. Clin. Sci. 13:59–66.

    CAS  Google Scholar 

  • Burd, J.F., Usategui-Gomez, M., Fernandez De Castro, A., Mhatre, N.S., and Yeager, F.M. (1973a). Immunochemical studies on lactate dehydrogenase: Preparation and characterization of anti-M lactate dehyrogenase antiserum: Development of an immunochemical assay for lactate dehydro genase isoenzymes in human serum. Clin. Chim. Acta. 46:205–215.

    Article  PubMed  CAS  Google Scholar 

  • Burd, J.F., and Usategui-Gomez, M. (1973b). Immunochemical studies on lactate dehydrogenase. Biochim. Biophys. Acta. 310:238–247.

    PubMed  CAS  Google Scholar 

  • Burgher, A., Richterich, H., and Aebi, H. (1964). Die Hetero genitat der Kreatin Kinase. Biochim. Z. 339:305–314.

    Google Scholar 

  • Cahn, R.D., Kaplan, N.O., Levin, L., and Zwilling, E. (1962). Nature and development of lactic dehydrogenase. Science 136:962–969.

    Article  PubMed  CAS  Google Scholar 

  • Fogh-Andersen, N., Sorensen, P., Muller-Petersen, J., and Ring, T. (1982). Lactate dehydrogenase isoenzyme I in diagnosis of myocardial infarction. J. Clin. Chem. Clin. Biochem. 20:291–294.

    PubMed  CAS  Google Scholar 

  • Foo, A.Y., Nemesanszky, E., and Rosalki, S.B. (1981). Comparison of procedures for the measurement of the anodal isoenzymes of lactate dehydrogenase in serum. Ann. Clin. Biochem. 18:232–236.

    PubMed  CAS  Google Scholar 

  • Galen, R.S., and Gambino, S.R. (1975). Isoenzymes of CPK and LDH in myocardial infarction and certain other diseases. Pathobiol. Annu. 5:283–315.

    PubMed  CAS  Google Scholar 

  • Galen, R.S. (1978). Isoenzymes and myocardial infarction. Diagn. Med. 1:40–52.

    Google Scholar 

  • Gerhardt, W., Hofuendahl, S., Ljungdahl, L., Waldenstrom, J., Tryding, N., Pettersson, T., and Ohlsson, O. (1983). Serum LD-1 activity in suspected acute myocardial infarction. Clin. Chem. 29:1057–1060.

    PubMed  CAS  Google Scholar 

  • Hall, N., Addis, P., and DeLuca, M. (1979). Mitochondrial creatine kinase. Physical and kinetic properties of the purified enzyme from beef heart. Biochem. 18:1745–1751.

    Article  CAS  Google Scholar 

  • Heinz, J.W., O’Donnel, N.J., and Lott, J.A. (1980). Apparent mitochondrial creatine kinase in the serum of a patient with metastatic cancer to the liver. Clin. Chem. 26:1908–1911.

    PubMed  CAS  Google Scholar 

  • James, G.P., and Harrison, R.L. (1979). Creatine kinase isoenzymes of mitochondrial origin in human serum. Clin. Chem. 25:943–947.

    PubMed  CAS  Google Scholar 

  • Jockers-Wretou, E. and Pfleiderer, G. (1975). Quantitation of creatine kinase isoenzymes in human tissues and sera by an immunological method. Clin. Chim. Acta. 58:223–232.

    Article  PubMed  CAS  Google Scholar 

  • Jockers-Wretou, E., and Plessing, E. (1979). Atypical serum creatine kinase isoenzyme pattern caused by complexing of creatine kinase-BB with immunoglobulins G and A. J. Clin. Clin. Biochem. 17:731–737.

    CAS  Google Scholar 

  • Keutel, H.J., Okabe, K., Jacobs, H.K., et al. (1972). Studies on adenosine triphosphate transphosphorylases XI. Isolation of the crystalline adenosine triphosphate creatine transphosphorylases from the muscle and brain of man, calf and rabbit, and a preparation of their enzymatically active hybrids. Arch. Biochem. Biophys. 150:648–678.

    Article  PubMed  CAS  Google Scholar 

  • Kwong, T.C., and Arvan, D.A. (1981). How many creatine kinase “isoenzymes” are there and what is their clinical significance? Clin. Chim. Acta. 115:3–8.

    Article  PubMed  CAS  Google Scholar 

  • Liu, F., Beiding, R., Usategui-Gomez, M., and Reynoso, G., (1981). Immunochemical determination of LDH-1. Amer. J. Clin. Path. 75:701–707.

    CAS  Google Scholar 

  • Lott, J.A., and Stang, J.M. (1980). Serum enzymes and isoenzymes in the diagnosis and differential diagnosis of myocardial ischemia and necrosis. Clin. Chem. 26:1241–1250 Review.

    PubMed  CAS  Google Scholar 

  • Ljungdahl, L., and Gerhardt, W. (1978). Creatine kinase isoenzyme variants in human serum. Clin. Chem. 24:832–834.

    PubMed  CAS  Google Scholar 

  • Markert, C.L., and Appell, E. (1963). Immunochemical properties of lactate dehydrogenase isoenzymes. Ann. N.Y. Acad. Sci. 103:915–929.

    Article  CAS  Google Scholar 

  • Marmor, A., Sobel, B.E., and Roberts, R. (1981). Factors presaging early recurrent myocardial infarct (“extension”) Amer. CI. Cardiol. 48:603–610.

    Article  CAS  Google Scholar 

  • McCoy, M.T., Aguanno, J.J., and Ritzmann, S.E. (1983). Lactate dehydrogenase isoenzyme I; A rapid assay for ischemic myocardial disease. Arch. Intern. Med. 143:434–436.

    Article  PubMed  CAS  Google Scholar 

  • Morin, L.B. (1979). Creatine kinase isoenzyme antibody reactions in immunoinhibition and immunonephelometry. Clin. Chem. 25:1415–1419.

    PubMed  CAS  Google Scholar 

  • Neumeier, D., Prellwitz, W., Wurzburg, U., et al. (1976). Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Clin. Chim. Acta. 73:445–451.

    Article  PubMed  CAS  Google Scholar 

  • Newman, E., Hager, H.J., and Hansen, H.J. (1980). Preparation of Kynar resin particles as a dispersed solid-phase antibody. Paper given at 72nd annual meeting of the Amer. Assoc, for Cancer Research, May 1980. Details of polyvinylidene fluoride coupling can be found in U.S. Patent No. 3,843,443.

    Google Scholar 

  • Nisselbaum, J.S. and Bodansky, O. (1963). Immunochemical studies of human lactic dehydrogenase. Ann. N.Y. Acad. Sci. 103:930–937.

    Article  PubMed  CAS  Google Scholar 

  • Pesce, M.A. (1982). The CK isoenzymes: Findings and their meaning. Lab. Mgmt. 20:25–37.

    Google Scholar 

  • Pesce, M., and Bodourian, S. (1983). Effect of atypical creatine kinase isoenzymes and CK-BB on an immunochemical CK- MB assay. Clin. Chem. 29:584–585.

    PubMed  CAS  Google Scholar 

  • Roberts, R., Parker, C.W., and Sobel, B.E. (1977). Detection of acute myocardial infarction by radioimmunoassay for creatine kinase MB. Lancet ii, 319–322.

    Article  Google Scholar 

  • Roberts, R. and Grace, A.M. (1980). Purification of mitochondrial creatine kinase. J. Biol. Chem. 255:2280–2287.

    Google Scholar 

  • Sax, S.M., Moore, J.J., Giegel, J.L., and Welsh, M. (1979). Further observations on the incidence and nature of atypical creatine kinase activity. Clin. Chem. 25:535–541.

    PubMed  CAS  Google Scholar 

  • Seckinger, D., Vasquez, A., Rosenthal, P., and Mendizabal, R. (1983). Cardiac isoenzyme methodology and the diagnosis of acute myocardial infarction. Amer. J. Clin. Path. 80:164–169.

    CAS  Google Scholar 

  • Stein, W., Bohner, J., Steinhart, R., and Eggstein, M. (1982). Macro creatine kinase: Determination and differentiation of two types by their activation energies. Clin. Chem. 28:19–24.

    PubMed  CAS  Google Scholar 

  • Usategui-Gomez, M., Wicks, R.W., and Warshaw, M. (1979). Immunochemical determination of the heart isoenzyme of lactate dehydrogenase (LDH.,) in human serum. Clin. Chem. 25:729–734.

    PubMed  CAS  Google Scholar 

  • Usategui-Gomez, M., Wicks, R., Farrenkopf, B., et al. (1981). Immunochemical determination of CK-MB isoenzyme in human serum: A radiometric approach. Clin. Chem. 27:823–827.

    PubMed  CAS  Google Scholar 

  • Vessell, E.S., and Beam, A.G. (1958). The heterogeneity of lactic and malic dehydrogenase. Ann. N.Y. Acad. Sci. 75:286–291.

    Article  Google Scholar 

  • Wang, T.Y., Godfrey, J.H., Graham, L.G., Haddad, M.N., and Hamilton, T.C. (1982). Clinical evaluation of immuno chemical assay of lactate dehydrogenase isoenzyme I in early detection of acute myocardial infarction. Clin. Chem. 28:2152–2154.

    PubMed  CAS  Google Scholar 

  • Weider, N. (1982). Laboratory diagnosis of acute myocardial infarction: Usefulness of determination of lactate dehydrogenase (LDH)-l level and of ratio of LDH-1 to total LDH. Arch. Pathol. Lab. Med. 106:375–377.

    Google Scholar 

  • Wevers, R., Mul-Steinbush, M., and Soons, J. (1980). Mitochondrial CK (EC 2.7.3.2) in the human heart. Clin. Chim. Acta. 101:103–111.

    Article  PubMed  CAS  Google Scholar 

  • Wicks, R.W., Usategui-Gomez, M., Miller, M., and Warshaw, M. (1982). Immunochemical determination of CK-MB .isoenzyme in human serum: II. An enzymic approach. Clin. Chem. 28:54–58.

    PubMed  CAS  Google Scholar 

  • Wu, A., and Bowers, G. (1982). Evaluation and comparison of immunoinhibition and immunoprecipitation methods for differentiating MB from BB and macro forms of creatine kinase isoenzymes in patients and healthy individuals. Clin. Chem. 28:2017–2021.

    PubMed  CAS  Google Scholar 

  • Yuu, H., Ishizawa, S., Takagii, Y., et al., (1980). Macro creatine kinase: A study on CK-linked immunoglobulin. Clin. Chem. 26:1816–1820.

    PubMed  CAS  Google Scholar 

  • Zweig, M.H., Van Steirteghem, A.C., and Schecter, A.N. (1978). Radioimmunoassay of creatine kinase isoenzymes in human serum: Isoenzyme BB. Clin. Chem. 24:422–428.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Diagnostic Research, Hoffmann-La Roche Inc., 340 Kingsland Street, Nutley, NJ, 07110, USA

    Richard Wicks & Magdalena Usategui-Gomez

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  1. Richard Wicks
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  2. Magdalena Usategui-Gomez
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Editors and Affiliations

  1. University of California, Irvine, Irvine, California, USA

    T. T. Ngo  & H. M. Lenhoff  & 

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© 1985 Plenum Press, New York

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Wicks, R., Usategui-Gomez, M. (1985). Development of Immunochemical Enzyme Assays for Cardiac Isoenzymes. In: Ngo, T.T., Lenhoff, H.M. (eds) Enzyme-Mediated Immunoassay. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5012-5_21

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  • DOI: https://doi.org/10.1007/978-1-4684-5012-5_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5014-9

  • Online ISBN: 978-1-4684-5012-5

  • eBook Packages: Springer Book Archive

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