Radial Partition Enzyme Immunoassay

  • Joseph L. Giegel


Radial Partition Immunoassay permits highly sensitive measurements of both low and high molecular weight ligands. The entire immunoassay is conducted on a small section of filter paper and assays can be done in less than 10 minutes. A variety of labels can be used with the technology including enzymes, fluorophores, chromophores or radioisotopes. We have achieved a high degree of sensitivity using enzyme labels and fluorescent readout of enzyme activity. The general principles of the radial partition enzyme immunoassay system are illustrated in Fig. 1.


Sandwich Assay Competitive Assay Sequential Assay Glass Fiber Filter Paper Hook Effect 
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  1. A.
    Giegel, J. L., Brotherton, M. M., and Cronin P. et al. 1982. Radial partition immunoassay. Clin. Chem. 28:1894–1898.PubMedGoogle Scholar
  2. B.
    Granick, S., (1942), Ferritin I physical and chemical properties of horse spleen ferritin. J. Biol. Chem. 24:451–461.Google Scholar
  3. C.
    Ishikawa, E., Imagawa, M., and Hashida, S. et al. 1983. Enzyme labeling of antibodies and their fragments for enzyme immunoassay. J. Immunoassay 4:209–327.PubMedCrossRefGoogle Scholar
  4. D.
    Miles, L.E.M., Lipschitz, D. A., Bieber, C. P., and Cook, J.D., 1974, Measurement of serum ferritin by a 2-site immunometric assay. Anal. Biochem. 61:209–224.PubMedCrossRefGoogle Scholar
  5. E.
    Ng, R. H. Brown, B. A., and Valdes, R. Jr., 1983. Three commercial methods for serum ferritin compared and the high dose “hook effect”eliminated, Clin. Chem. 25: 326–329.Google Scholar
  6. F.
    Rodbard, N., 1978, Statistical estimation of the minimal detectable concentration (“sensitivity”) for radioligand assay. Anal, Biochem. 90:1–12.CrossRefGoogle Scholar
  7. G.
    Smith, T. W., Butler, V. P., and Haber, E., 1970, Characterization of antibodies of high affinity and specificity for the digitalis glycoside digoxin. Biochem, 2:331.Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Joseph L. Giegel
    • 1
  1. 1.American Dade CompanyAmerican Hospital Supply Corp.MiamiUSA

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