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Steric Hindrance Enzyme Immunoassay (Sheia)

  • Albert Castro
  • Nobuo Monji

Abstract

We have developed a new enzyme immunoassay for the quantitation of small antigens. The assay is called SHEIA (Steric Hindrance Enzyme Immunoassay) since, this assay involves the interference of complex formation between the antigen (or hapten) coupled enzyme and the enzyme inhibitor attached to agarose by antibody to the antigen (or hapten), probably due to steric hindrance (Monji and Castro, 1979). The assay principle is shown in Figure 1.

Keywords

Affinity Chromatography Separation Step Normal Rabbit Serum Detectable Enzyme Activity Alpha Omega 
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References

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Albert Castro
    • 1
  • Nobuo Monji
    • 2
  1. 1.Department of PathologyUniversity of Miami School of MedicineMiamiUSA
  2. 2.Genetic Systems CorporationSeattleUSA

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