Separation-Required (Heterogenous) Enzyme Immunoassay for Haptens and Antigens

  • Jim C. Standefer


Immunoassays that require separation of bound antigen from the unbound fraction have been developed for a large variety of haptens and antibodies (Avrameas,1969; Voller et al., 1976; Engvall and Carlsson,1976; O’Sullivan et al., 1979a). These immunoassay techniques may be divided into two catagories, i.e., sequential (noncompetitive) and competitive. The sequential, heterogenous immunoassay technique, otherwise known as the Enzyme Linked Immunosorbent Assay or ELISA, has been most widely adopted.


Enzyme Immunoassay Serum Ferritin ELISA Assay Sulfhydryl Group Cyanogen Bromide 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Jim C. Standefer
    • 1
  1. 1.Department of PathologyUniversity of New Mexico School of MedicineAlbuquerqueUSA

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