Advertisement

Introduction

  • T. T. Ngo
  • H. M. Lenhoff

Abstract

In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon a competition between insulin labeled with radioactive iodine (I131) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA).

Keywords

Radioactive Iodine Unbind Fraction FEBS Letter Heterogeneous Phase Immunoenzymatic Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Avrameas, S., P. Druet, R. Masseyeff, and G. Feldmann, Eds. (1983). Immunoenzymatic Techniques. Elsevier Science Publisher, Amsterdam.Google Scholar
  2. Engvall, E. and P. Perlmann (1971). Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochem. 8: 871–874.CrossRefGoogle Scholar
  3. Ishikawa, E., T. Kawai and K. Miyai, Eds. (1981). Enzyme Immunoassay. Igaku-Shoin, Tokyo.Google Scholar
  4. Malvano, R., Ed. (1980). Immunoenzymatic Assay Techniques, Martinus Nijhoff Publisher, The Hague.Google Scholar
  5. Van Weemen, B. K. and A. H. W. M. Schuurs (1971). Immunoassay using antigen-enzyme conjugate. FEBS Letters. 15: 232–236.PubMedCrossRefGoogle Scholar
  6. Yalow, R. S. and S. A. Berson (1959). Assay of plasma insulin in human subjects by immunological methods. Nature 184: 1648–1649.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • T. T. Ngo
    • 1
  • H. M. Lenhoff
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

Personalised recommendations