The Transforming Genes of Polyoma Virus

  • Minoo Rassoulzadegan
  • Francois Cuzin
Part of the NATO ASI Series book series (NSSA, volume 91)


As indicated by its name, polyoma virus induces a variety of histologically different tumors (Stewart, 1960). Like its close relative SV40, the virus is in fact able to transform a large spectrum of differenciated rodent cells. Most commonly used target cells are rodent (rat, mouse, hamster) fibroblasts: two types of cultures are available, the most normal primary cultures derived from 12–15 day rodent embryos (REF cells) and the established (3T3) lines, which, although in principle non tumorigenic, have acquired one of the characters of a transformed cell, the ability to grow in culture for an indefinite number of generations (Todaro & Green, 1963). Transformation of REF or 3T3 cells leads to the same “highly transformed” phenotype: as originally shown by the pioneer work of Vogt & Dulbecco (1962,1963), these cells grow in all cases indefinitely in culture, grow in culture past confluency, in suspension, in the absence of serum factors, exhibit a completely disorganized cytoskeleton, etc. These cell lines are highly tumorigenic in the syngeneic animal.


Polyoma Virus Cellular Oncogene Syngeneic Animal Host Range Mutant Rodent Embryo 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Minoo Rassoulzadegan
    • 1
  • Francois Cuzin
    • 1
  1. 1.Unité de Génétique Moléculaire des Papovavirus (INSERM U273), Centre de BiochimieUniversité de Nice, Parc ValroseNiceFrance

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