The Transforming Genes of Polyoma Virus
As indicated by its name, polyoma virus induces a variety of histologically different tumors (Stewart, 1960). Like its close relative SV40, the virus is in fact able to transform a large spectrum of differenciated rodent cells. Most commonly used target cells are rodent (rat, mouse, hamster) fibroblasts: two types of cultures are available, the most normal primary cultures derived from 12–15 day rodent embryos (REF cells) and the established (3T3) lines, which, although in principle non tumorigenic, have acquired one of the characters of a transformed cell, the ability to grow in culture for an indefinite number of generations (Todaro & Green, 1963). Transformation of REF or 3T3 cells leads to the same “highly transformed” phenotype: as originally shown by the pioneer work of Vogt & Dulbecco (1962,1963), these cells grow in all cases indefinitely in culture, grow in culture past confluency, in suspension, in the absence of serum factors, exhibit a completely disorganized cytoskeleton, etc. These cell lines are highly tumorigenic in the syngeneic animal.
KeywordsPolyoma Virus Cellular Oncogene Syngeneic Animal Host Range Mutant Rodent Embryo
Unable to display preview. Download preview PDF.
- 2).CUZIN, F. (1984). The polyoma virus oncogenes: coordinated functions of three distinct proteins in the transformation of rodent cells in culture. Biochim. Biophys. Acta, 781, 193.Google Scholar
- 7).ITO, Y. (1980). Organization and expression of the genome of polyoma virus. In: Viral Oncology (ed. G. Klein ), p. 447, Raven Press, NY.Google Scholar
- 9).MOUGNEAU, E., LEMIEUX, L., RASSOULZADEGAN, M., & CUZIN, F. (1984). Biological activities of v-myc and rearranged c-myc oncogenes in rat fibroblast cells in culture. Proc. Natl. Acad. Sci. USA, in press.Google Scholar
- 11).RASSOULZADEGAN, M., GAUDRAY, P., CANNING, M., TREJO-AVILA, L., & CUZIN, F. (1981). Two polyoma gene functions involved in the expression of the transformed phenotype in FR3T3 rat cells. I. Localization of a transformation maintenance function in the proximal half of the large-T coding region. Virology, 114, 489.CrossRefGoogle Scholar
- 16).SEIF, R. & CUZIN, F. (1977). Temperature-sensitive growth regulation in one type of transformed rat cells induced by the tsa mutant of polyoma virus. J. Virol., 24, 721.Google Scholar
- 20).TYNDALL, C., LA MANTIA, G., THACKER, C.M., FAVALORO, J., & KAMEN, R. (1981). A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication. Nucl. Acids Res., 9, 6231.CrossRefGoogle Scholar