Advertisement

Cyclin (PCNA) is a Component of the Pathway(s) Leading to DNA Replication and Cell Division: A Role in DNA Replication?

  • Julio E. Celis
  • Ariana Celis
Part of the NATO ASI Series book series (NSSA, volume 91)

Abstract

Understanding of the molecular mechanisms underlying malignant transformation and cancer will be assisted by the identification of cellular proteins whose activity may be involved in the control of cell proliferation in normal cells (1–3 and references therein). The acidic nuclear polypeptide cyclin (Mr=36,000; IEF 49 in the HeLa protein catalogue, 4–6; see also Fig. 1) is potentially such a candidate as the levels of this protein are modulated during the cell cycle (increase in S-phase; 7) and correlate directly with the proliferative state of normal cells. Cyclin is presend in very small amounts in normal non-dividing cells (senescent and quiescent cells included) and tissues, but is synthesized by proliferating cells both of normal and transformed origin, tumours included (1,7–25). So far, most of the properties of cyclin are also shared by the proliferating cell nuclear antigen (PCNA; 26–29), a human protein that has recently been shown to be identical with cyclin (21).

Keywords

Proliferate Cell Nuclear Antigen Staining Pattern Immunofluorescence Study Cervical Adenocarcinoma Putative Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    CELIS, J.E., BRAVO, R., MOSE LARSEN, P., FEY, S.J., BELLATIN, J., & CELIS, A. (1984). In: Two-Dimensional Gel Electrophoresis of Proteins: Methods and Applications (eds J.E. Celis & R. Bravo), p. 308, Academic Press, New York.Google Scholar
  2. 2).
    BISHOP, J.M. (1983). Ann. Rev. Biochem., 52, 301.CrossRefGoogle Scholar
  3. 3).
    LEVINE, A., TOPP, W., VAN DE WOUDE, G., & WATSON, J.D. eds (1984). Cancer Cells, Cold Spring Harbor Laboratory.Google Scholar
  4. 4).
    BRAVO, R., BELLATIN, J., & CELIS, J.E. (1981). Cell Biol. Int. Rep., 5, 93.CrossRefGoogle Scholar
  5. 5).
    BRAVO, R. & CELIS, J.E. (1982). Clin. Chem. (Winston-Salem, NC) 28, 766.Google Scholar
  6. 6).
    BRAVO, R. & CELIS, J.E. (1984). In: Two-Dimensional Gel Electrophoresis of Proteins: Methods and Applications (eds J.E. Celis & R. Bravo), p. 445, Academic Press, New York.Google Scholar
  7. 7).
    BRAVO, R. & CELIS, J.E. (1980). J. Cell Biol., 48, 795.CrossRefGoogle Scholar
  8. 8).
    BRAVO, R. & CELIS, J.E. (1980). Exp. Cell Res., 127, 249.CrossRefGoogle Scholar
  9. 9).
    BRAVO, R., FEY, S.J., BELLATIN, J., MOSE LARSEN, P., AREVALO, J., & CELIS, J.E. (1981). Exp. Cell Res., 136, 311.CrossRefGoogle Scholar
  10. 10).
    BRAVO, R., FEY, S.J., & CELIS, J.E. (1981). Carcinogenesis, 2, 769.CrossRefGoogle Scholar
  11. 11).
    CELIS, J.E. & BRAVO, R. (1981). Trends Biochem. Sci., 6, 197.Google Scholar
  12. 12).
    BRAVO, R., FEY, S.J., BELLATIN, J., MOSE LARSEN, P., & CELIS, J.E. (1982). In: Embryonic Development (ed. M. Burger ), Part A, p. 235.Google Scholar
  13. 13).
    BRAVO, R. & CELIS, J.E. (1982). Clin. Chem. (Winston-Salem, NC), 28, 949.Google Scholar
  14. 14).
    BELLATIN, J., BRAVO, R., & CELIS, J.E. (1982). Proc. Natl. Acad. Sci. USA, 79, 4367.CrossRefGoogle Scholar
  15. 15).
    BRAVO, R., BELLATIN, J., FEY, S.J., MOSE LARSEN, P., & CELIS, J.E. (1983). In: Gene Expression in Normal and Transformed Cells (eds J.E. Celis & R. Bravo), p. 263, Plenum, New York.Google Scholar
  16. 16).
    FORCHHAMMER, J. & MACDONALD-BRAVO, H. (1983). In: Gene Expression in Normal and Transformed Cells (eds J.E. Celis & R. Bravo), p. 291, Plenum, New York.Google Scholar
  17. 17).
    CELIS, J.E. & BRAVO, R. (1984). FEBS Lett., 165, 21.CrossRefGoogle Scholar
  18. 18).
    BRAVO, R. (1984). FEBS Lett., 169, 185.CrossRefGoogle Scholar
  19. 19).
    CELIS, J.E., BRAVO, R., MOSE LARSEN, P., & FEY, S.J. (1984). Leuk. Res., 8, 143.CrossRefGoogle Scholar
  20. 20).
    CELIS, J.E., FEY, S.J., MOSE LARSEN, P., & CELIS, A. (1984). Proc. Natl. Acad. Sci. USA, 81, 3128.CrossRefGoogle Scholar
  21. 21).
    MATHEWS, M.B., BERNSTEIN, R.M., FRANZA, R., & GARRELS, J.I. (1984). Nature, 309, 374.CrossRefGoogle Scholar
  22. 22).
    CELIS, J.E., FEY, S.J., MOSE LARSEN, P., & CELIS, A. (1984). Cancer Cells, 1, 123.Google Scholar
  23. 23).
    FRANZA, B.R. & GARRELS, J.I. (1984). Cancer Cells, 1, 137.Google Scholar
  24. 24).
    BRAVO, R. (1984). Cancer Cells, 1, 147.Google Scholar
  25. 25).
    BRAVO, R. (1984). Proc. Natl. Acad. Sci. USA, 81, 4848.CrossRefGoogle Scholar
  26. 26).
    MIYACHI, K., FRITZLER, M.J., & TAN, E.M. (1978). J. Immunol., 121, 2228.Google Scholar
  27. 27).
    TAKASAKI, Y., DENG, J.S., & TAN, E.M. (1981). J. Exp. Med., 154, 1899.CrossRefGoogle Scholar
  28. 28).
    TAN, E.M. (1982). Adv. Immunol., 33, 167.CrossRefGoogle Scholar
  29. 29).
    TAKASAKI, Y., FISCHWILD, D., & TAN, E.M. (1984). J. Exp. Med., 159, 981.CrossRefGoogle Scholar
  30. 30).
    CELIS, J.E. & CELIS, A. (1985). Proc. Natl. Acad. Sci. USA, in press.Google Scholar
  31. 31).
    CELIS, J.E. & BRAVO, R. (1984). In: Electrophoresis ‘84 (ed. V. Neuhoff), p. 205, Verlag Chemie.Google Scholar
  32. 32).
    WILLIAMS, C.A. & OCKEY, C.H. (1970). Exp. Cell Res., 63, 365.CrossRefGoogle Scholar
  33. 33).
    YANISHEVSKY, R.M. & PRESCOTT, D.M. (1978). Proc. Natl. Acad. Sci USA, 75, 3307.CrossRefGoogle Scholar
  34. 34).
    CELIS, J.E. & CELIS, A., in preparation.Google Scholar
  35. 35).
    TAYLOR-PAPADIMITRIOU, J., PURKIS, P., LANE, E.B., MCKAY, I.A., & CHANG, S.E. (1982). Cell Differ., 11, 169.Google Scholar
  36. 36).
    QUINLAN, M.P., BO CHEN, L., & KNIPE, D.M. (1984). Cell, 36, 857.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Julio E. Celis
    • 1
  • Ariana Celis
    • 1
  1. 1.Division of Biostructural Chemistry, Department of ChemistryAarhus UniversityAarhus CDenmark

Personalised recommendations