Functional Domains of Purified Adenovirus Type C E1A Proteins
The adenovirus E1A gene controls initial stages of oncogenic transformation (1–5) and activates transcription of a variety of viral and host cell genes (6–11). We have a strong interest in the molecular details of these important regulatory functions. For this reason, some of us (12) have inserted the E1A coding sequence in a prokaryotic expression vector in order to generate the quantities of pure E1A proteins that are required to study their action. Here, we summarize results, to be published in detail elsewhere, which have allowed us to define more closely those sequences of the E1A gene that determine the intracellular location of E1A proteins as well as their functions during the virus life cycle.
KeywordsVirus Life Cycle Nuclear Movement Adenovirus Type Prokaryotic Expression Vector Important Regulatory Function
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