Production and Characterization of Anti-Morphine Anti-Idiotypic Antibodies
Anti-morphine antibodies (AM-Ab’s) have now been generated in rabbits by immunization with 3-0-carboxymethylmorphine-bovine serum albumin (BSA). The polyclonal antibodies (PAb’s) mimicked the opioid binding characteristics of opiate receptors since they bound morphine stereospecifically and with high affinity. Anti-idiotypic antibodies (anti-Id Ab’s) which cross-react with the opiate receptor were produced by immunizing guinea pigs and mice with purified AM-Ab. Guinea pig antisera produced opiate-like responses in the isolated ileal longitudinal muscle and vas deferens preparations. With antisera present the binding of [3H]naloxone to mouse-brain homogenate was reduced. Scatchard analysis indicated that its binding was to two different sites, each affected by antisera. Anti Id antisera administered to mice attenuated biological responses to opiates.
KeywordsOpiate Receptor Male Hartley Guinea Naloxone Binding Immunological Image Ileal Longitudinal Muscle
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