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Anti-Receptor Antibodies by the Auto-Anti-Idiotypic Route

  • B. F. Erlanger
  • W. L. Cleveland
  • N. H. Wassermann
  • B. L. Hill
  • A. S. Penn
  • H. H. Ku
  • R. Sarangarajan
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)

Abstract

A procedure was developed for preparing antibodies (Ab’s) to the acetylcholine receptor (AChR) on the supposition that, regardless of functional differences, macromolecules of the same specificity will show binding-site homologies. Ab’s prepared in rabbits to a structurally constrained agonist of AChR (BisQ) mimicked the binding specificity of AChR in its activated state; agonist binding affinities parallelled biological activity. Rabbits immunized with a specifically purified preparation of anti-BisQ produced anti-idiotypic (anti-Id) Ab’s that cross-reacted with determinants on AChR preparations from Torpedo californica, Electrophorus electricus and rat muscle. Moreover, some rabbits showed signs of transient experimental myasthenia gravis, manifesting circulating AChR Ab’s.

Keywords

Booster Injection Internal Image Idiotypic Network Receptor Preparation Idiotypic Determinant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • B. F. Erlanger
    • 1
  • W. L. Cleveland
    • 1
  • N. H. Wassermann
    • 1
  • B. L. Hill
    • 1
  • A. S. Penn
    • 2
  • H. H. Ku
    • 1
  • R. Sarangarajan
    • 1
  1. 1.Departments of MicrobiologyColumbia UniversityNew YorkUSA
  2. 2.Neurobiology and the Cancer Center/Institute of Cancer ResearchColumbia UniversityNew YorkUSA

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