Antibody-Screening cDNA Libraries
Molecular cloning and ancillary techniques offer an attractive alternative in studying many biological phenomena. Successful molecular cloning of genes encoding proteins for which amino acid sequence data are not available can be achieved by antibody (Ab) screening. Requisite precautions in adopting immunological screening methods include interaction of Ab preparations with host bacterial proteins (i.e. host bacteria for plasmid uptake). This should be assessed by Western blotting of host bacterial proteins or by performing immunoprecipitations following in vivo Labelling of the host bacterial proteins.
KeywordsA431 Cell cDNA Library Xenopus Oocyte Antigen Preparation Hybrid Selection
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