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Antibody-Screening cDNA Libraries

  • Frank A. Simmen
  • Denis R. Headon
  • Tanya Z. Schulz
  • Mohan Cope
  • David A. Wright
  • Graham Carpenter
  • Bert W. O’Malley
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)

Abstract

Molecular cloning and ancillary techniques offer an attractive alternative in studying many biological phenomena. Successful molecular cloning of genes encoding proteins for which amino acid sequence data are not available can be achieved by antibody (Ab) screening. Requisite precautions in adopting immunological screening methods include interaction of Ab preparations with host bacterial proteins (i.e. host bacteria for plasmid uptake). This should be assessed by Western blotting of host bacterial proteins or by performing immunoprecipitations following in vivo Labelling of the host bacterial proteins.

Keywords

A431 Cell cDNA Library Xenopus Oocyte Antigen Preparation Hybrid Selection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Frank A. Simmen
    • 1
  • Denis R. Headon
    • 2
  • Tanya Z. Schulz
    • 1
  • Mohan Cope
    • 1
  • David A. Wright
    • 3
  • Graham Carpenter
    • 4
  • Bert W. O’Malley
    • 1
  1. 1.Department of Cell BiologyBaylor College of MedicineHoustonUSA
  2. 2.Department of BiochemistryUniversity CollegeGalwayIreland
  3. 3.Department of GeneticsM.D. Anderson Hospital and Tumor InstituteHoustonUSA
  4. 4.Department of BiochemistryVanderbilt University School of MedicineNashvilleUSA

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