Investigating the Specificity of Monoclonal Antibodies to Protein Antigens Using β-Galactosidase Fusion Proteins

  • D. P. Lane
  • J. Gannon
  • S. P. J. Brennan
  • S. E. Mole
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)


Numerous monoclonal antibodies (MAb’s) to the oncogene, large T, of the small double-stranded DNA (dsDNA) tumour virus SV40 have been produced [1–4, and J. Yewdell & D.P.Lane, unpublished work). Detailed analysis of these Ab’s* has provided insights into both the structure and the function of large T, besides protein-antigen immunochemistry. The Ab’s can be subdivided by the following criteria.— (a) Ability of the Ab to recognize the protein after denaturation. (b) Recognition of only a sub-set of the T antigen molecules. (c) Inhibition of biological activity of large T. (d) Location of the binding site of the Ab on the linear structure and the 3-dimensional structure of the protein. Each subdivision can be enhanced by subcloning fragments of the large-T coding information into bacterial and eukaryotic expression vectors that cause the large-T fragment to be incorporated at the carboxy terminus of β-galactosidase [5, 6].


Fusion Protein Protein Antigen Carboxy Terminus Eukaryotic Expression Vector Cancer Research Campaign 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • D. P. Lane
    • 1
  • J. Gannon
    • 1
  • S. P. J. Brennan
    • 1
  • S. E. Mole
    • 1
  1. 1.Department of Biochemistry Cancer Research Campaign Eukaryotic Molecular Genetics Research GroupImperial CollegeLondonUK

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