An Approach to the Study of Anti-Protein Antibody Combining Sites

  • M. J. Darsley
  • P. de la Paz
  • D. C. Phillips
  • A. R. Rees
  • B. J. Sutton
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)


The main aims of this study are to investigate the nature of the immune response to a single defined antigenic region of a protein and to explore in atomic detail the interaction between antibody (Ab) and a protein antigen. Virtually no structural data are yet available for anti-protein Ab combining sites, since X-ray crystallographic studies have hitherto been restricted to myeloma proteins that bind small-molecule haptens, and only recently have structural studies of monoclonal antibodies (MAb’s) to protein antigens been undertaken [1, 2]. Here we describe briefly a procedure for modelling Ab combining sites based on sequence data alone. This approach has been applied to a series of MAb’s and their complexes with a single antigenic region of the lysozyme molecule.


Protein Antigen Antigenic Region Myeloma Protein Epitope Residue Lysozyme Molecule 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • M. J. Darsley
    • 1
  • P. de la Paz
    • 1
  • D. C. Phillips
    • 1
  • A. R. Rees
    • 1
  • B. J. Sutton
    • 1
  1. 1.Laboratory of Molecular Biophysics Department of ZoologyUniversity of OxfordOxfordUK

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