An Approach to the Study of Anti-Protein Antibody Combining Sites
The main aims of this study are to investigate the nature of the immune response to a single defined antigenic region of a protein and to explore in atomic detail the interaction between antibody (Ab) and a protein antigen. Virtually no structural data are yet available for anti-protein Ab combining sites, since X-ray crystallographic studies have hitherto been restricted to myeloma proteins that bind small-molecule haptens, and only recently have structural studies of monoclonal antibodies (MAb’s) to protein antigens been undertaken [1, 2]. Here we describe briefly a procedure for modelling Ab combining sites based on sequence data alone. This approach has been applied to a series of MAb’s and their complexes with a single antigenic region of the lysozyme molecule.
KeywordsProtein Antigen Antigenic Region Myeloma Protein Epitope Residue Lysozyme Molecule
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- 5.Darsley, M.J. and Rees, A.R. (1985) EMBO J. 4, 383–392.Google Scholar
- 7.Saul, F., Amzel, L. and Poljak, R. (1978) J. Biol. Chem. 253, 585–597.Google Scholar
- 13.Novotny, J., Bruccoleri, R., Newell, J., Murphy, D., Haber, E. and Karplus, M. (1983) J. Biol. Chem. 258, 14433–14437.Google Scholar
- 16.Darsley, M.J. and Rees, A.R. (1985) EMBO J. 4, 393–398.Google Scholar
- 20.Aschaffenburg, R., Phillips, D.C., Rose, D.R., Sutton, B.J., Dower, S.K. and Dwek, R.A. (1979) Biochem. J. 181, 497–499.Google Scholar