An Approach to the Study of Anti-Protein Antibody Combining Sites
The main aims of this study are to investigate the nature of the immune response to a single defined antigenic region of a protein and to explore in atomic detail the interaction between antibody (Ab) and a protein antigen. Virtually no structural data are yet available for anti-protein Ab combining sites, since X-ray crystallographic studies have hitherto been restricted to myeloma proteins that bind small-molecule haptens, and only recently have structural studies of monoclonal antibodies (MAb’s) to protein antigens been undertaken [1, 2]. Here we describe briefly a procedure for modelling Ab combining sites based on sequence data alone. This approach has been applied to a series of MAb’s and their complexes with a single antigenic region of the lysozyme molecule.
KeywordsAlbumin Myeloma Lysozyme Biot Photography
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