Comparative Structures of Mouse Antibody Combining Sites

  • David R. Davies
  • Talapady N. Bhat
  • Gerson H. Cohen
  • Eduardo A. Padlan
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)


The crystal structures of two mouse myeloma antibody-binding fragments (Fab’s) have been determined: McPC603, an anti-phosphocholine immunoglobulin (anti-PC Ig), and J539 which is specific for galactan. A comparison has been made between the sequences of other mouse Ig’s that have similar binding properties. In both heavy and light chains of the PC-binding proteins, the residues in contact with the PC remain invariant or highly conserved. Examination of the pattern of charge conservation at the combining site reveals a possible coupling of somatic mutations in order to maintain specificity.


Light Chain Heavy Chain Central Cavity Chain Sequence Amino Acid Sequence Information 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Köhler, G. & Milstein, C. (1975) Nature 256, 495–497.CrossRefGoogle Scholar
  2. 2.
    Silverton, E.W., Davies, D.R., Smith-Gill, S. & Potter, M. (1983) Abs. Am. Crystallographic Assn. 11 (2), 28.Google Scholar
  3. 3.
    Mariuzza, R.A., Jankovic, D.L., Boulot, G., Amit, A.G., Saludjian, P., LeGuern, A., Mazie, J.C. & Poljak, R.J. (1983) J. Mot. Biol. 170, 1055–1058.CrossRefGoogle Scholar
  4. 4.
    Bernstein, F.C., Koetzke, T.F., Williams, G.J.B., Meyer, E.F., Brice, M.D., Rogers, J.R., Kennard, O., Shimanouchi, T. & Tasumi, M. (1977) J. Mol. Biol. 112, 535–542.CrossRefGoogle Scholar
  5. 5.
    Padlan, E.A., Segal, D.M., Rudikoff, S., Potter, M., Spande, T.F. & Davies, D.R. (1973) Nature New Biol. 245, 165–167.Google Scholar
  6. 6.
    Segal, D.M., Padlan, E.A., Cohen, G.H., Rudikoff, S., Potter, M. & Davies, D.R. (1974) Proc. Nat. Acad. Sci. 71, 4298–4302.CrossRefGoogle Scholar
  7. 7.
    Padlan, E.A., Davies, D.R., Rudikoff, S. & Potter, M. (1976) Immunochemistry 13, 945–949.CrossRefGoogle Scholar
  8. 8.
    Davies, D.R. & Metzger, H. (1983) Ann. Rev.Immunol.1,87–117.Google Scholar
  9. 9.
    Kabat, E.A., Wu, T.T., Bilofsky, H., Reid-Miller, M. & Perry, H. (1983) Sequences of Proteins of Immunological Interest, National Institutes of Health, Bethesda, MD. [Cf. E.A. Kabat, this vol.-Ed.]Google Scholar
  10. 10.
    Gearhart, P., Johnson, N.D., Douglas, R. & Hood, L. (1981) Nature 291, 29–34.CrossRefGoogle Scholar
  11. 11.
    Rudikoff, S. (1983) in Contemporary Topics in Molecular Immunology, Vol. 9 (Inman, F.P. & Kindt, T.J., eds.), Plenum, New York, pp. 169–209.Google Scholar
  12. 12.
    Navia, M.A., Segal, D.M., Padlan, E.A., Davies, D.R., Rao, D.N., Rudikoff, S. & Potter, M. (1979) Proc. Nat. Acad. Sci. 76, 4071–4074.CrossRefGoogle Scholar
  13. 13.
    Saul, F., Amzel, L.M. & Poljak, R.J. (1978) J. Biol. Chem. 253, 585–597.Google Scholar
  14. 14.
    Silverton, E.W., Navia, M.A. & Davies, D.R. (1977) Proc. Nat. Acad. Sci. 74, 5140 - -5144.Google Scholar
  15. 15.
    Marquart, M., Deisenhofer, J., Huber, R. & Palm, W. (1980) J. Mol. Biol. 141, 369–391.CrossRefGoogle Scholar
  16. 16.
    Manjula, B.N. & Glaudemans, C.P.J. (1976) Immunochemistry 13, 469–471.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • David R. Davies
    • 1
  • Talapady N. Bhat
    • 1
  • Gerson H. Cohen
    • 1
  • Eduardo A. Padlan
    • 1
  1. 1.Laboratory of Molecular BiologyNational Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of HealthBethesdaUSA

Personalised recommendations