Advertisement

Ca2+-Binding Proteins Located on the Cytoplasmic Face of the Lymphocyte Plasma Membrane

  • Raymond J. Owens
  • Adelina A. Davies
  • Christopher J. Gallagher
  • Mark Hexham
  • Michael J. Crumpton
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)

Abstract

Three polypeptides of mol. wts.~68,000, 33,000 and 28,000 have been identified in Ca2+ -chelator extracts of purified preparations of lymphocyte plasma membrane (p.m.). They are preferentially associated with the actin-rich, Nonidet P40-insoluble fraction of the membrane and are not labelled by lactoperoxidase-catalyzed iodination of whole lymphocytes, suggesting that they are located on the cytoplasmic face of the p.m. The 68,000 mol. wt. protein, the most abundant of the three in the EGTA extract, has been purified from pig and human lymphocyte p.m. and partially characterized. It is a monomeric acidic protein with a single high-affinity Ca2+-binding site (KD = 1.2 μM). From their solubility with Ca2+ -chelators present, the 33,000 and 28,000 components seem to be Ca2+-binding proteins too.

Keywords

Polypeptide Composition Cytoplasmic Face Human Tonsil Imperial Cancer Research Fund Burkitt Lymphoma Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Metzger, H. (1978) Immunol. Rev. 41, 186–199.CrossRefGoogle Scholar
  2. 2.
    Salisbury, J.L., Condeelis, J.S., Satir, P. (1980) J. Cell Biol. 87, 132–141.CrossRefGoogle Scholar
  3. 3.
    Kretsinger, R.H. (1979) Adv. Cyclic Nucleotide Res. 11, 1–26.Google Scholar
  4. 4.
    Yu, J., Fishman, D.A., Steck, T.L. (1973) J. Supramol. Struct. 1, 233–248.CrossRefGoogle Scholar
  5. 5.
    Davies, A.A., Wigglesworth, N.M., Allan, D., Owens, R.J., Crumpton, M.J. (1984) Biochem. J. 219, 301–308.Google Scholar
  6. 6.
    Mescher, M.F., Jose, M.J., Balk, S.P. (1981) Nature 289, 139–141.CrossRefGoogle Scholar
  7. 7.
    Crumpton, M.J., Snary, D. (1974) Contemp. Top. Mol. Immunol. 3, 27–56.CrossRefGoogle Scholar
  8. 8.
    Snary, D., Woods, F.R., Crumpton, M.J. (1976) Anal. Biochem. 74, 457–465.CrossRefGoogle Scholar
  9. 9.
    Glenney, J.R., Glenney, P. (1983) Cell 34, 503–517.CrossRefGoogle Scholar
  10. 10.
    Bretscher, M.S., Raff, M.C. (1975) Nature 258, 43–49.CrossRefGoogle Scholar
  11. 11.
    Owens, R.J., Crumpton, M.J. (1984) Biochem. J. 219, 309–316.Google Scholar
  12. 12.
    Owens, R.J., Gallagher, C.J., Crumpton, M.J. (1984) EMBO J. 3, 945–952.Google Scholar
  13. 13.
    Lenk, R., Penman, S. (1979) Cell 16, 289–301.CrossRefGoogle Scholar
  14. 14.
    Greenberg, M.E., Edelman, G.M. (1983) Cell 33, 767–779.CrossRefGoogle Scholar
  15. 15.
    Lehto, V.-P., Virtanen, I., Passivuo, R., Ralston, R., Alitalo, K. (1983) EMBO J. 2, 1701–1705.Google Scholar
  16. 16.
    Nigg, E.A., Cooper, J.A., Hunter, T. (1983) J. Cell Biol. 96, 1601–1609.CrossRefGoogle Scholar
  17. 17.
    Erickson, E., Erickson, R.L. (1980) Cell 21, 829–836.CrossRefGoogle Scholar
  18. 18.
    Sudhof, T.C., Ebbecke, M., Walker, J.R., Fritzsche, U., Boustead, C. (1984) Biochemistry 23, 1103–1109.CrossRefGoogle Scholar
  19. 19.
    Gerke, V., Weber, K. (1984) EMBO J. 3, 227–233.Google Scholar
  20. 20.
    Courtneidge, S., Ralston, R., Alitalo, K., Bishop, J.M. (1983) Mol. Cell. Biol. 3, 340–350.Google Scholar
  21. 21.
    Gould, K.L., Cooper, J.A., Hunter, T. (1984) J. Cell Biol. 98, 487–497.CrossRefGoogle Scholar
  22. 22.
    Greenberg, M.E., Brackenbury, R., Edelman, G.M. (1984) J. Cell Biol. 98, 473–485.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Raymond J. Owens
    • 1
  • Adelina A. Davies
    • 1
  • Christopher J. Gallagher
    • 2
  • Mark Hexham
    • 1
  • Michael J. Crumpton
    • 1
  1. 1.Imperial Cancer Research FundLincoln’s Inn FieldsLondonUK
  2. 2.Royal Marsden HospitalLondonUK

Personalised recommendations