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Use of Polyclonal and Monoclonal Antibodies in Studying Complement Component C9 as a Trans-Membrane Protein

  • J. Paul Luzio
  • Keith K. Stanley
  • Peter Jackson
  • Kenneth Siddle
  • B. Paul Morgan
  • Anthony K. Campbell
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)

Abstract

C9 is a serum protein that acts as the final component of the complement membrane attack complex (MAC). For its purification, assay, and immunofluorescent localization in biopsy specimens, use has been made of 5 mouse monoclonal antibodies (MAb’s) that recognize different antigenic sites. The Ab’s have also been used to show that C9 becomes a transmembrane protein on insertion into the MAC, and one Ab has been used to identify a cloned cDNA coding for C9 in a bacterial expression vector. With proteolytic cleavage and immuno­blotting, along with functional inhibition experiments, the folding of C9 through the target membrane is being mapped.

Keywords

Antigenic Site Membrane Attack compLex Hybrid Protein Bacterial Expression Vector Short Open Reading Frame 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • J. Paul Luzio
    • 1
  • Keith K. Stanley
    • 2
  • Peter Jackson
    • 1
  • Kenneth Siddle
    • 1
  • B. Paul Morgan
    • 3
  • Anthony K. Campbell
    • 3
  1. 1.Department of Clinical BiochemistryUniversity of Cambridge Addenbrooke’s HospitalCambridgeUK
  2. 2.European Molecular Biology LaboratoryHeidelbergW. Germany
  3. 3.Department of Medical BiochemistryUniversity of Wales College of MedicineCardiffUK

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