Intermediate Filament Diversity as Detected by Antibodies
Based on biochemical and immunological properties, 5 cell type-specific iF classes have been identified  (each having a central α-helical rod portion flanked by non-α-helical head and tail regions), according to their subunit protein(s): #Ck’s, 2–10/cell (40−68 × 103 apparent mol. wt. by SDS-PAGE) in ep’l and ep’1-derived cells; #Vm (57) in non-ep’l cells, esp. mesenchymal, and in some ep’l cell cultures; #desmin (53) in muscle cells; #GFAP (51) in astrocytes and gliomas; #neurofilament proteins, 3/cell (210, 160 & 68) in neurons and related tumours. Co-localization reflects co-polymerization, with molecular proximity, in two cases (Vm-desmin, Vm-GFAP ). PAb’s and MAb’s specific for each iF class are important in histology and especially in tumour diagnosis [3, 4]. There are as many as ~20 Ck’s , the set depending on the ep’l type  — as exploited in tumour and cell-line typing . By various criteria including antigenic determinants , Ck’s fall into 2 groups which each contribute to an iF-assembly pair: type I, acidic; type II, more basic with i.e.p. in 9.5 M urea up to 8 (vs. ~5.5 for all other iF types). Purification of iF’s relies on nonextractability by (e.g.) Triton-X-100. Solubilization of iF proteins needs an agent such as citric or acetic acid, SDS, or buffer rich in urea or guanidine [9,10].
KeywordsMitotic Cell Interphase Cell MDBK Cell Filament Staining Cytoplasmic Dense Body
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