An Enzyme-Linked Immunosorbent Assay for Calmodulin
Calmodulin (CaM) is a Ca2+-binding protein notably important in the translation of Ca2+ signals inside the cell (as reviewed [1, 2]) [cf. A.R. Means & J.G. Chafouleas, Vol. 13, #A-7, this series — Ed.]. It is found throughout the plant and animal kingdoms and its sequence is highly conserved . Measurement of CaM by its activation of 3’,5’-cyclic nucleotide phosphodiesterase, and using radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA), provides useful comparative measurements of the amount of protein and its activity. Methods have been described for assay by enzyme activation  and by RIA ([5, 6], & Vol. 13 art. cited above). Here we describe the production of an anti-CaM antibody (Ab) and its use in setting up an ELISA. Elsewhere  we exemplify the applicability of the ELISA.
KeywordsAmmonium Sulphate Cyclic Nucleotide Phosphodiesterase DEAE Column Polystyrene Cuvette Cuvette Wall
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