An Enzyme-Linked Immunosorbent Assay for Calmodulin

  • M. E. Bardsley
  • P. J. Roberts
  • I. R. Cottingham
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)


Calmodulin (CaM) is a Ca2+-binding protein notably important in the translation of Ca2+ signals inside the cell (as reviewed [1, 2]) [cf. A.R. Means & J.G. Chafouleas, Vol. 13, #A-7, this series — Ed.]. It is found throughout the plant and animal kingdoms and its sequence is highly conserved [3]. Measurement of CaM by its activation of 3’,5’-cyclic nucleotide phosphodiesterase, and using radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA), provides useful comparative measurements of the amount of protein and its activity. Methods have been described for assay by enzyme activation [4] and by RIA ([5, 6], & Vol. 13 art. cited above). Here we describe the production of an anti-CaM antibody (Ab) and its use in setting up an ELISA. Elsewhere [7] we exemplify the applicability of the ELISA.


Ammonium Sulphate Cyclic Nucleotide Phosphodiesterase DEAE Column Polystyrene Cuvette Cuvette Wall 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • M. E. Bardsley
    • 1
  • P. J. Roberts
    • 1
  • I. R. Cottingham
    • 2
  1. 1.Department of Physiology and Pharmacology Medical & Biological SciencesUniversity of SouthamptonSouthamptonUK
  2. 2.Department of BiochemistryUniversity of SussexFalmer, BrightonUK

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