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Immunological Approaches and Techniques Employed in Tubulin and Microtubule Research

  • Christopher R. Birkett
  • Keith Gull
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)

Abstract

It was through the use of indirect immunofluorescence (IF) using monospecific polyclonal antisera that it was first possible to visualize in their entirety the arrays of microtubules within cells. Now, by using specific monoclonal antibodies (MAb’s), it is possible to distinguish subsets of microtubule populations within these arrays. Some MAb’s bind only to species-specific tubulins and are even capable of defining individual electrophoretic tubulin isotypes within the full spectrum of tubulin polypeptides produced by a single organism. Because it is possible to exactly determine the nature of the binding site of a MAb, it becomes a probe for molecular structure, the sensitivity and specificity of which has not previously been available. As the library of MAb’s expands it should therefore be possible to investigate the specific protein constituents of microtubules and the nature of their interaction with many other cellular organelles. Such work is still in its comparative infancy, but already there are examples of how this approach will be able to benefit our understanding of the microtubules within the cell.

Keywords

Cell Biol Polyclonal Antiserum Immunological Approach Cytoplasmic Microtubule Vimentin Filament 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Christopher R. Birkett
    • 1
  • Keith Gull
    • 1
  1. 1.Biological LaboratoryUniversity of KentCanterbury, KentUK

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