Effect of Multivalent Binding on Dissociation of Monoclonal Antibodies from Cell Surface Antigens
Clearance of antibodies (Ab’s) from cell surfaces has been studied in vitro with two IgG monoclonal Ab’s (11/160 and 12/FCIII) directed against surface antigens of the rat fibrosarcoma HSN.TC. When Ab-coated HSN.TC cells were incubated in fresh medium at 37°, 12/FCIII was lost rapidly (8% of the initial binding remaining after 2 h), whereas 11/160 was cleared relatively slowly (85% of the initial binding remaining after 10 h). Gel-filtration analysis of radiolabelled Ab shed into the supernatant medium over the first 5 h showed the majority (>80%) of the recovered activity as intact IgG, with the remainder as low-mol. wt. fragments. Loss of radiolabelled monoclonal Ab from the cells was progressively inhibited by treatment of monoclonal-Ab-coated cells with increasing concentrations of antiglobulin before incubation at 37°, and was accompanied by a greater output of degraded fragments.
KeywordsCell Surface Similar Condition Fresh Medium Surface Antigen Early Observation
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