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Human:Mouse Antibody-Producing Transfectoma Cell Lines

  • Vernon T. Oi
  • Sherie L. Morrison
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)

Abstract

A new means to generate antibodies (Ab’s) with desired antigen-specificities and biological effector functions is to create transfectoma Ab-producing cell lines. These cell lines are derived from lymphoid cell lines that have been transfected with novel immunoglobulin genes using DNA-mediated gene transfer techniques. The context is our attempts at understanding the regulation of immunoglobulin gene expression. A direct outcome of these studies has been a means of generating human Ab molecules with mouse antigen-specificities.

Keywords

Calcium Phosphate Myeloma Cell Immunoglobulin Gene Calcium Phosphate Precipitate Lymphoid Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Herzenberg, Leonore A., Hayakawa, K., Hardy, R.R., Tokuhisa, T., Oi, V.T. and Herzenberg, Leonard A. (1982) Immunol. Rev. 67, 5–31.CrossRefGoogle Scholar
  2. Oi, V.T., Vuong, T.M., Hardy, R., Reidler, J., Dangl, J., Herzenberg, L.A. and Stryer, L. (1984) Nature 307, 136–140.CrossRefGoogle Scholar
  3. Sharon, J., Gefter, M.L., Manser, T., Morrison, S.L., Oi, V.T. and Ptashne, M. (1984) Nature 309, 364–367.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Vernon T. Oi
    • 1
  • Sherie L. Morrison
    • 2
  1. 1.Becton-DickinsonMonoclonal CenterMoutain ViewUSA
  2. 2.Department of Microbiology and the Cancer Center, Institute for Cancer Research, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

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