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Monoclonal Internal Image Anti-Idiotypic Antibodies of Hepatitis B Surface Antigen

  • Yasmin M. Thanavala
  • Angela Bond
  • Richard Tedder
  • Frank C. Hay
  • Ivan M. Roitt
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 15B)

Abstract

Using a monoclonal antibody (Ab) to hepatitis B surface antigen (HBsAg) we have raised 6 monoclonal anti-idiotypic Ab’s. If the anti-idiotype is behaving as an ‘internal image’ of HBsAg, then, like antigen it should bind polyclonal anti-HBsAg sera raised in different species where it is less likely that idiotypes unrelated to antigen binding would be identical. Using polyclonal goat, swine, rabbit and human anti-HBsAg sera in an indirect immunofluorescence technique we have shown that two of the hybridomas stained strongly (Fig. 1). No staining was seen with various control sera or with hybridomas of unrelated specificities.

Keywords

Antigen Binding Control Seron Polyclonal Goat Hospital Medical School Internal Image 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Yasmin M. Thanavala
    • 1
  • Angela Bond
    • 1
  • Richard Tedder
    • 2
  • Frank C. Hay
    • 1
  • Ivan M. Roitt
    • 1
  1. 1.Department of ImmunologyThe Middlesex Hospital Medical SchoolLondonUK
  2. 2.Department of VirologyThe Middlesex Hospital Medical SchoolLondonUK

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