Intrastriatal Grafting of Dopamine-Containing Cell Suspensions: Effects of Mixing with Target and Non-Target Cells
Grafting of dissociated cell suspensions prepared from embryonic ventral mesencephalon (VM), rich in dopamine (DA) containing cells, into 6-hydroxydopamine (6-OHDA) denervated rat striatum has previously been shown to survive and restore some functional deficits induced by the lesion (Björklund et al., 1983). Dissociated VM cells allowed to reaggregate and develop in rotatory cultures in vitro have a greater number of surviving DA-cells when co-aggregated with tissue from a DA target area, i.e. the striatal primordium (Hoffman et al., 1983, Shalaby et al., 1984) as compared to cultures of DA cells alone or of DA cells reaggregated with non-target cells. In addition, in vitro culturing of DA neurons mixed with striatal target cells has been shown to induce an increase DA-synthesis and uptake (Di Porzio et al., 1980) as well as induce changes in the morphology of the DA neurons (Denis-Donini et al., 1983). The present experiment was designed to investigate to what extent the addition of embryonic striatal target cells to VM cell suspensions might influence the survival, fibre outgrowth and functional effects of VM cell suspension grafts in the denervated striatum of adult host rats.