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Electron Paramagnetic Resonance in Biology: Spin Trapping

  • Alexandre T. Quintanilha
Part of the NATO ASI Series book series (NSSA, volume 91)

Abstract

Discovered in 1945 by Zavoisky1, electron paramagnetic resonance (EPR) has since been applied to a large number of research areas. It is a spectroscopic method using frequencies in the microwave region (i.e. 109 up to 1011hz) and wavelengths from approximately 10−3 to 10−1 meters; it is limited to the detection of unpaired electrons. Unpaired electrons are present in free radicals, triplet electronic states and transition and rare earth ions. The sensitivity of the method allows radical concentrations of 10 nM to be detected.

Keywords

Electron Paramagnetic Resonance Applied Magnetic Field Unpaired Electron Apparent Rate Constant Spin Trap 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    E. Zavoiski, J. Phys. U.S.S.R. Vol. 9, p. 211 (1945).Google Scholar
  2. 2.
    M. J. Perkins, in “Essays in Free Radical Chemistry” p.97, Special Publication No.24, Chemical Society London, 1970.Google Scholar
  3. 3.
    E. G. Janzen, C. A. Evans, and E. R. Davis, in “Organic Free Radicals” p. 433 (ACS Symposium Series No. 69) American Chemical Society, 1978.Google Scholar
  4. 4.
    G. M. Rosen and E. J. Rauckman “Spin Trapping of Superoxide and Hydroxyl Radicals” in Methods of Enzymology (ed. S. P. Colowick and N. O. Kaplan) Vol. 105, p. 198, 1984.Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Alexandre T. Quintanilha
    • 1
    • 2
  1. 1.Department of Physiology-AnatomyUniversity of CaliforniaBerkeleyUSA
  2. 2.Departamento de Biofisica Instituto Abel SalazarUniversidade do PortoPortoPortugal

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