The Nature of Proteins in Membranes
The first part of this article concerns a central theme which I have been pursuing for many years. It is the demonstration that the secret of biological systems rests in the connection
Chemical Composition → Structure → Mobility → Function
By analysis: its composition and sequence.
By X-ray diffraction: its structure in a particular trapped state in a crystal. The dynamics of the structure in the crystal can add some useful knowledge, but relatively little because of the short time constants observed (10−12sec).
The mobility of the structure in solution as determined by NMR largely but with help from many spectroscopic tools. In essence this says that X-ray structures are unlikely to provide a unique answer to the states (plural) available to the protein.
Function implies that there may be states not open to direct inspection under (2) and (3) but which may be inferred from (2) and (3) plus a knowledge of the activity of the protein.
KeywordsEpidermal Growth Factor Receptor Fast Electron Transfer Short Time Constant Helical Protein Single Helix
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