Drug Biotransformation and Drug Toxicity Studied with Intact Mammalian Cells
Since many years our laboratory has been actively engaged in studies of biochemical mechanisms associated with drug biotransformation and drug toxicity. Our studies were originally concerned with the properties and regulation of the major enzymes involved in drug metabolism, in particular the cytochrome P-450 monooxygenase system. More recently, our research has been focused on biochemical mechanisms of importance for development of drug toxicity, and the role of the glutathione system as a major cellular defense mechanism. In these studies we have used a variety of experimental model systems, including perfused tissues, suspensions of intact cells, subcellular organelle fractions, and purified enzymes. This presentation summarizes some of our more recent studies of drug biotransformation and drug toxicity, using predominantly freshly isolated rat hepatocytes to investigate the underlying biochemical mechanisms.
KeywordsDrug Toxicity Redox Cycling Pyridine Nucleotide Protein Thiol Semiquinone Radical
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