The Micronucleus Test on Exfoliated Human Cells
The frequency of micronuclei in exfoliated human or animal cells can be used as an “endogenous dosimeter” present in tissues which are targets for carcinogenic and genotoxic agents and from which carcinomas arise. The application of the micronucleus test to exfoliated cells of various human tissues will provide evidence of exposure to carcinogens and clastogens, a measure of the degree of genotoxic damage, and an estimate of additive or enhancing effects when several carcinogens or genotoxic agents act together. To a certain degree, this test may be used to identify population groups at high risk for cancer. It is advisable to make duplicate smears of exfoliated cells. One set should be stained with the Feulgen reaction and used for scoring the frequency of micronuclei. The second set should be stained according to Papanicolau and used for cytopathological diagnosis. A combined application of the micronucleus test and cytopathological analysis will provide information on the frequency of genotoxic aberrations at various preneoplastic stages, including metaplasia, dysplasia, and carcinomas in situ.
KeywordsMicronucleus Test Genotoxic Agent Schistosoma Haematobium Smear Preparation Micronucleated Cell
Unable to display preview. Download preview PDF.
- Rosin, M. P., and Stich, H. F., 1983, The identification of anti-genotoxic/anticarcinogenic agents in food, in: “Diet and Cancer: From Basic Research to Policy Implications” D. A. Roe, ed., Alan R. Liss, New York, pp. 141–154.Google Scholar
- Stich, H. F., and Rosin, M. P., 1983, Micronuclei in exfoliated human cells as an internal dosimeter for exposures to carcinogens, in: “Carcinogens and Mutagens in the Environment,” Vol. II, “Naturally Occurring Compounds: Endogenous Formation and Modulation,” H. F. Stich, ed., CRC Press, Boca Raton, Florida, pp. 17–25.Google Scholar