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The Micronucleus Test on Exfoliated Human Cells

  • H. F. Stich
  • W. Stich
  • M. P. Rosin
Part of the Basic Life Sciences book series

Abstract

The frequency of micronuclei in exfoliated human or animal cells can be used as an “endogenous dosimeter” present in tissues which are targets for carcinogenic and genotoxic agents and from which carcinomas arise. The application of the micronucleus test to exfoliated cells of various human tissues will provide evidence of exposure to carcinogens and clastogens, a measure of the degree of genotoxic damage, and an estimate of additive or enhancing effects when several carcinogens or genotoxic agents act together. To a certain degree, this test may be used to identify population groups at high risk for cancer. It is advisable to make duplicate smears of exfoliated cells. One set should be stained with the Feulgen reaction and used for scoring the frequency of micronuclei. The second set should be stained according to Papanicolau and used for cytopathological diagnosis. A combined application of the micronucleus test and cytopathological analysis will provide information on the frequency of genotoxic aberrations at various preneoplastic stages, including metaplasia, dysplasia, and carcinomas in situ.

Keywords

Micronucleus Test Genotoxic Agent Schistosoma Haematobium Smear Preparation Micronucleated Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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General References

  1. Rosin, M. P., and Stich, H. F., 1983, The identification of anti-genotoxic/anticarcinogenic agents in food, in: “Diet and Cancer: From Basic Research to Policy Implications” D. A. Roe, ed., Alan R. Liss, New York, pp. 141–154.Google Scholar
  2. Stich, H. F., and Rosin, M. P., 1983, Quantitating the synergistic effect of smoking and alcohol consumption with the micronucleus test on human buccal mucosa cells, Int. J. Cancer, 31:305–308.PubMedCrossRefGoogle Scholar
  3. Stich, H. F., and Rosin, M. P., 1983, Micronuclei in exfoliated human cells as an internal dosimeter for exposures to carcinogens, in: “Carcinogens and Mutagens in the Environment,” Vol. II, “Naturally Occurring Compounds: Endogenous Formation and Modulation,” H. F. Stich, ed., CRC Press, Boca Raton, Florida, pp. 17–25.Google Scholar
  4. Stich, H. F., and Rosin, M. P., 1984, Micronuclei in exfoliated human cells as a tool for studies in cancer risk and cancer intervention, Cancer Lett., 22:241–253.PubMedCrossRefGoogle Scholar
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  6. Stich, H. F., San, R. H. C., and Rosin, M. P., 1983, Adaptation of the DNA repair and micronucleus tests to human cell suspensions and exfoliated cells, Ann. N.Y. Acad. Sci., 407:93–105.PubMedCrossRefGoogle Scholar
  7. Stich, H. F., Stich, W., and Parida, B. B., 1982, Elevated frequency of micronucleated cells in the buccal mucosa of individuals at high risk for oral cancer: betel quid chewers, Cancer Lett., 17:125–134.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • H. F. Stich
    • 1
  • W. Stich
    • 1
  • M. P. Rosin
    • 1
  1. 1.Environmental Carcinogenesis UnitBritish Columbia Cancer Research CentreVancouverCanada

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