Advertisement

Mutagen Testing with Yeast

  • Friederike Eckardt
  • Wolfram Siede
Part of the Basic Life Sciences book series

Abstract

This article deals primarily with the practical aspects of mutagen testing with yeast. Equipment necessary for a laboratory where mutagen testing with yeast is performed, and the most commonly used media, are listed. Some general procedures are described and, finally, for those who have little experience with work of this kind, a precise protocol is given for an experiment with stationary phase cells of the strain D7 of Saccharomyces cerevisiae using the heteroallelic ade2 system as the genetic endpoint. Some experimental data were obtained by students following this protocol using the direct-acting mutagen ethyl methanesulfonate (EMS); these data are discussed and analyzed.

More details on the various genetic endpoints available in numerous yeast strains and on the interpretation of dose-dependence data, as well as an extended list of yeast literature, can be found in an article by Eckardt and von Borstel in this volume. Further technical advice is provided in our references to Zimmermann (1975), von Borstel (1981), and Zimmermann et al. (1984).

Keywords

Mutagen Testing Ethyl Methane Sulfonate Stationary Phase Cell Ethyl Methane Sulfonate Logarithmic Phase Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arnes, B. N., McCann, J., and Yamasaki, E, 1975, Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test, Mutat. Res., 31:347–364.Google Scholar
  2. Callen, D. F., and Philpot, R. M., 1977, Cytochrome P-450 and the activation of promutagens in Saccharomyces cerevisiae, Mutat. Res., 45:309–324.PubMedCrossRefGoogle Scholar
  3. de Serres, F. J., and Ashby, J., 1981, “Evaluation of Short-Term Tests for Carcinogens,” Elsevier/North-Holland, New York, 827 pages.Google Scholar
  4. Eckardt, F., and Haynes, R. H., 1977, Kinetics of mutation induction by ultraviolet light in excision-deficient yeast, Genetics, 85:225–247.PubMedGoogle Scholar
  5. Eckardt, F., and von Borstel, R. C., 1985, Mutagen testing of agricultural chemicals with yeast, in: “Basic and Applied Mutagenesis: with Special Reference to Agricultural Chemicals in Developing Countries,” Amir Muhammed and R. C. von Borstel, eds., Plenum Press, New York, pp. 221–248.Google Scholar
  6. Haynes, R. H., and Eckardt, F., 1980, Mathematical analysis of mutation induction kinetics, in: “Chemical Mutagens,” Vol. 6, A. Hollaender and F. J. de Serres, eds., Plenum Press, New York, pp. 271–307.CrossRefGoogle Scholar
  7. Kelly, D., and Parry, J. M., 1983, Metabolic activation of cytochrome P-450/P-448 in the yeast Saccharomyces cerevisiae, Mutat. Res., 108:147–159.PubMedCrossRefGoogle Scholar
  8. Parry, J. M., and Sharp, D., 1981, Induction of mitotic aneuploidy in the yeast strain D6 by 42 coded compounds, in: “Evaluation of Short-Term Tests for Carcinogens,” F. J. de Serres and J. Ashby, eds., Elsevier/North-Holland, New York, pp. 468–480.Google Scholar
  9. von Borstel, R. C., 1981, The yeast Saccharomyces cerevisiae: An assay organism for environmental mutagens, in: “Short-Term Tests for Assaying Mutagens,” H. F. Stich and R. H. C. San, eds., Springer-Verlag, New York, pp. 161–174.CrossRefGoogle Scholar
  10. Zimmermann, F. K., 1973, A yeast strain for visual screening for the two reciprocal products of mitotic crossing-over, Mutat. Res., 21:263–269.PubMedCrossRefGoogle Scholar
  11. Zimmermann, F. K., 1975, Procedures used in the induction of mitotic recombination and mutation in the yeast Saccharomyces cerevisiae, Mutat. Res., 31:71–86.PubMedGoogle Scholar
  12. Zimmermann, F. K., and Scheel, I., 1981, Induction of mitotic gene conversion in strain D5 of Saccharomyces cerevisiae by 42 coded compounds, in: “Evaluation of Short-Term Tests for Carcinogens” F. J. de Serres and J. Ashby, eds., Elsevier/ North-Holland, New York, pp. 481–490.Google Scholar
  13. Zimmermann, F. K., Kern, R., and Rasenberger, H., 1975, A yeast strain for simultaneous detection of induced mitotic crossing-over, mitotic gene conversion and reverse mutation, Mutat. Res., 28:381–188.CrossRefGoogle Scholar
  14. Zimmermann, F. K., von Borstel, R. C., Von Halle, E. S., Parry, J. M., Siebert, D., Zetterberg, G., Barale, R., and Loprieno, N., 1984, Testing of chemicals for genetic activity with Saccharomyces cerevisiae: a report of the U.S. Environmental Protection Agency Gene-Tox Program, Mutat. Res., 133:199–244.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Friederike Eckardt
    • 1
  • Wolfram Siede
    • 1
  1. 1.Abteilung für StrahlenbiologieGesellschaft für Strahlen- und UmweltforschungNeuherbergFederal Republic of Germany

Personalised recommendations