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Genetic Engineering: Principles and Methods pp 135–148Cite as

Analysis of Chromosome Replication with Eggs of Xenopus Laevis

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Part of the book series: Genetic Engineering: Principles and Methods ((GEPM,volume 7))

Abstract

Although eukaryotic transcription systems have advanced dramatically in the last decade, systems for studying the other main function of the cell nucleus, chromosome replication, have lagged far behind. Studies of chromosome replication in eukaryotes are still limited by a shortage of efficient experimental systems. Although some of the events involved in movement of the replication fork can be reproduced in vitro, there is still an urgent need for eukaryotic cell-free systems which initiate replication efficiently on purified duplex DNA templates.

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Author information

Author notes

  1. M. Mechali

    Present address: Institut Jacques Monod, Université Paris 7, 2 Place Jussieu, 75251, Paris Cedex 05, France

Authors and Affiliations

  1. CRC Molecular Embryology Group, Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK

    R. A. Laskey, S. E. Kearsey & M. Mechali

Authors
  1. R. A. Laskey
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  2. S. E. Kearsey
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  3. M. Mechali
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Editor information

Editors and Affiliations

  1. Brookhaven National Laboratory, Upton, New York, USA

    Jane K. Setlow

  2. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Alexander Hollaender

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© 1985 Plenum Press, New York

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Laskey, R.A., Kearsey, S.E., Mechali, M. (1985). Analysis of Chromosome Replication with Eggs of Xenopus Laevis . In: Setlow, J.K., Hollaender, A. (eds) Genetic Engineering: Principles and Methods. Genetic Engineering: Principles and Methods, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4973-0_6

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  • DOI: https://doi.org/10.1007/978-1-4684-4973-0_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4975-4

  • Online ISBN: 978-1-4684-4973-0

  • eBook Packages: Springer Book Archive

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