In Situ Hybridization to Cellular RNAs
A decade has passed since the first report of the detection of an mRNA by in situ hybridization (1, 2). In the past five years progress in two areas has greatly increased the utility of this technique. First, the technology of molecular genetics has made available a large number of gene sequences for use as probes for specific cellular and viral mRNAs. Second, work by a number of different investigators has led to the development of in situ hybridization techniques for an increasing variety of biological materials. These efforts have provided characterizations of the technique with respect to important experimental variables, increased the sensitivity of detection, and documented the specificity of the method. In situ hybridization is one of few methods which permit examination of gene expression at the resolution of single cells. It has demonstrated or potential applications ranging from basic problems in molecular genetics and developmental biology to use in diagnostic medicine.
KeywordsTarget RNAs Hybridization Efficiency Actin mRNA Symmetric Probe Hybridiza Tion
Unable to display preview. Download preview PDF.
- 32.Haase, A.T., Stowring, L., Geballe, A., Blum, H. and Ventura, P. (1984) Methods Virol. (in press).Google Scholar
- 41.Cox, K.H. (1983) Ph.D. Dissertation, University of Rochester, Rochester, NY.Google Scholar
- 44.Angerer, R.C. and Davidson, E.H. (1984) Science, November 12, 1984 (in press).Google Scholar
- 46.Showman, R.M., Wells, D.E., Anstrom, J.A., Hursh, D.A., Leaf, D.S. and Raff, R.A. (1983) in Molecular Aspects of Early Development (Malacinski, G.M. and Klein, W.H., eds.) pp. 109–130, Plenum Press, New York, NY.Google Scholar