Immunoscreening λgtll Recombinant DNA Expression Libraries

  • Richard A. Young
  • Ronald W. Davis
Part of the Genetic Engineering: Principles and Methods book series (GEPM, volume 7)


Most strategies used to isolate genes from recombinant DNA libraries have employed nucleic acid probes (1,2). Genes can also be identified and isolated through the use of antibody probes of the antigen(s) expressed by specific genes (3). This manuscript reviews the observations which led to the development of a system for efficient gene isolation using antibody probes, includes protocols for constructing and screening λgtll recombinant DNA expression libraries and describes strategies which can be used to confirm gene identity.


Cold Spring Harbor Foreign Antigen Nitrocellulose Filter Recombinant Phage Good Aeration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Richard A. Young
    • 1
  • Ronald W. Davis
    • 2
  1. 1.Whitehead Institute for Biomedical Research and Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Department of BiochemistryStanford University School of MedicineStanfordUSA

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