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Transcription of Cloned Eukaryotic Ribosomal RNA Genes

  • B. Sollner-Webb
  • J. Tower
  • V. Culotta
  • J. Windle
Part of the Genetic Engineering: Principles and Methods book series (GEPM, volume 7)

Abstract

A typical eukaryotic cell devotes about half of its transcriptional capacity to the synthesis of only one kind of RNA — this is the 40 to 47S ribosomal RNA (rRNA), precursor to the mature 18S, 28S and 5.8S RNAs of the ribosome. The transcription of ribosomal DNA (rDNA) is both very efficient and highly regulated, reflecting the cellular need to produce more than a million new ribosomes per generation which are required for protein synthesis.

Keywords

Initiation Site Tissue Culture Cell Primer Extension Analysis rRNA Synthesis rDNA Transcription 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • B. Sollner-Webb
    • 1
  • J. Tower
    • 1
  • V. Culotta
    • 1
  • J. Windle
    • 1
  1. 1.Department of Biological ChemistryJohns Hopkins University School of MedicineBaltimoreUSA

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