The Isolation and Characterization of the Blym-1 Transforming Gene

  • Alan Diamond
  • Joan M. Devine
  • Mary-Ann Lane
  • Geoffrey M. Cooper
Part of the Basic Life Sciences book series


The study of the genetic and molecular events which are involved in tumor development has been stimulated by the observation of discrete genes with oncogenic potential. Such genes have been detected in the genomes of acutely transforming retroviruses which induce tumors in susceptible hosts within a relatively short latent period (reviewed by Cooper, 1982). The mutation or deletion of such genes from the retroviral genome results in their loss of tumorigenicity. In addition, the ability of subgenomic fragments of retroviral DNA to induce the morphological transformation of recipient cells by transfection has provided further evidence for their role in neoplastic diseases (Anderson et al., 1979; Blair et al., 1980; Chang et al., 1980; Copeland et al., 1980; Barbacid, 1981). Sequences homologous to retroviral transforming genes have been detected in normal cellular DNA, suggesting that these sequences were acquired during the evolution of the viruses (Bishop, 1981), The first evidence that cellular DNA from neoplasms could efficiently transform recipient cells was reported by Shih et al. in 19 79 who showed that the high molecular weight DNA of chemically transformed mouse fibroblasts could transform other mouse cells by transfection.


Transforming Gene Burkitts Lymphoma Cell Line Subgenomic Fragment Harvey Murine Sarcoma Virus Bursal Lymphoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Andersson, P., Goldfarb, M.P, and Weinberg, R.A., 1979, A defined subgenomic fragment of in vitro synthesized Moloney-sarcoma virus DNA can induce cell transformation upon transfection, Cell, 16:63.PubMedCrossRefGoogle Scholar
  2. Barbacid, M., 1981, Cellular transformation by subgenomic feline sarcoma virus DNA, J. Virol., 37:518PubMedGoogle Scholar
  3. Bishop, J.M., 1981, Enemies within: The genesis of retrovirus oncogenes, Cell, 23:5.PubMedCrossRefGoogle Scholar
  4. Blair, D.G., McClements, W.L., Oskarsson, M.K., Fischinger, P.J., and Wande Woude, G.F., 1980, Biological activity of cloned Moloney sarcoma virus DNA: Terminally redundant sequences may enhance transformation efficiency, Proc. Natl. Acad. Sci. USA, 77:3504.PubMedCrossRefGoogle Scholar
  5. Chang, E.H., Maryak, J.M., Wei, C.-M., Shih, T.Y., Shober, R., Cheung, H.L., Ellis, R.W., Hager, G.L., Scolnick, E.M., and Lowy, D.R., 1980, Functional organization of the Harvey murine sarcoma virus genome, J. Virol., 35:76.PubMedGoogle Scholar
  6. Cooper, G.M., 1982, Cellular transformiîxg genes, Science, 217:801.PubMedCrossRefGoogle Scholar
  7. Cooper, G.M., and Lane, M.-A., 1984, Cellular transforming genes and oncogenesis, Biochem. Biophys. Acta, in press.Google Scholar
  8. Cooper, G.M., and Neiman, P.E., 1980, Transforming genes of neoplasms induced by avian lymphoid leukosis viruses, Nature (London), 287:656.CrossRefGoogle Scholar
  9. Cooper, G.M., and Neiman, P.E., 1981, Two distinct candidate transforming genes of lymphoid leukosis virus-induced neoplasms, Nature (London), 292:857.CrossRefGoogle Scholar
  10. Copeland, N.G., Zelentz, A.D., and Cooper, G.M., 1980, Transformation by subgenomic fragments of Rous sarcoma virus DNA, Cell, 19:863.PubMedCrossRefGoogle Scholar
  11. Dalla-Favera, R., Gregni, M., Erikson, J., Patterson, D., Gallo, R.C., and Croce, CM., 1982, Human c-myc one gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells, Proc. Natl. Acad. Sci. USA, 79:7824.PubMedCrossRefGoogle Scholar
  12. Diamond, A., Cooper, G.M., Ritz, J., and Lane, M.-A., Identification and molecular cloning of the human Blym transforming gene activated in Burkitt1s lymphomas, Nature (London), 305:112.Google Scholar
  13. Diamond, A., Devine, J.M., and Cooper, G.M., 1984, Nucleotide sequence of a human Blym transforming gene activated in a Burkitt’s lymphoma, Science, 225:516.PubMedCrossRefGoogle Scholar
  14. Goubin, G., Goldman, D.S., Luce, J., Neiman, P.E., and Cooper, G.M., 1983, Molecular cloning and nucleotide sequence of a transforming gene detected by transfection of chicken B-cell lymphoma DNA, Nature (London), 302:114.CrossRefGoogle Scholar
  15. Haynes, B.F., Hemler, M., Cotner, T., Mann, D.L., Eisenbarth, G.S., Strominger, J., and Fauci, A.S., 1981, Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation, J. Immunol., 127:347.PubMedGoogle Scholar
  16. Hayward, W.S., Neel, B.G., and Astrin, S.M., 1981, Activation of a cellular one gene by promoter insertion in ALV-induced lymphoid leukosis, Nature (London), 290:475.CrossRefGoogle Scholar
  17. Klein, G., 1981, The role of gene dosage and genetic transpositions in carcinogenesis, Nature (London), 294:313.CrossRefGoogle Scholar
  18. Lane, M.-A., Sainten, A., Doherty, K.M., and Cooper, G.M., 1984, Isolation and characterization of a stage-specific transforming gene, Tlym-I, from T-cell lymphomas, Proc. Natl. Acad. Sci. USA, 81:2227.PubMedCrossRefGoogle Scholar
  19. Macgilliray, R.T.A., Mendez, E., and Brew, K., 1977, in: “Proteins of Iron Metabolism,” E.B. Brown, P. Aisen, J. Fielding, and R.R. Crichton, eds., pp. 133–141, Grune and Stratton, New York.Google Scholar
  20. Manolov, G., and Manolova, Y.,. 1972, Marker band in one chromosome 14 from Burkitt lymphomas, Nature (London), 237:33.CrossRefGoogle Scholar
  21. Marcu, K.B., Harris, L.J., Stanton, L.W., Erikson, J., Watt, R., and Croce, CM., 1983, Transcriptionally active c-myc oncogene is contained within NIARD, a DNA sequence associated with chromosome translocations in B-cell neoplasia, Proc. Natl. Acad. Sci. USA, 80:519.PubMedCrossRefGoogle Scholar
  22. Morton, C.C., Taub, R., Diamond, A., Lane, M.-A., Cooper, G.M., and Leder, P., 1984, Mapping of the human Blym-1 transforming gene activated in Burkitt lymphomas to chromosome 1, Science, 223:173.PubMedCrossRefGoogle Scholar
  23. Neel, B.G., Jhanwar, S.C., Changant, R.S.K., and Hayward, W.S., 1982, Two human c-onc genes are located on the long arm of chromosome 8, Proc. Natl. Acad. Sci. USA, 79:7842.PubMedCrossRefGoogle Scholar
  24. Rowley, J., 1982, Identification of constant chromosome regions involved in human hematologic malignant disease, Science, 216:749.PubMedCrossRefGoogle Scholar
  25. Shih, C., Shilo, B.-Z., Goldbarb, M.P., Dannenberg, A., and Weinberg, R.A., 1979, Passage of phenotypes of chemically transformed cells via transfection of DNA and chromatin, Proc. Natl. Acad. Sci. USA, 76:5714.PubMedCrossRefGoogle Scholar
  26. Sutherland, R., Delia, D., Schneider, C., Newman, R., Kemshead, J., and Greaves, M., 1981, Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin, Proc. Natl. Acad. Sci. USA, 78:4515.PubMedCrossRefGoogle Scholar
  27. Taetle, R., Honeysett, J.M., and Trowbridge, I.S., 1983, Effects of anti-transferrin receptor antibodies on growth of normal and malignant myeloid cells, Int. J. Cancer, 32:343.PubMedCrossRefGoogle Scholar
  28. Taub, R., Kirsch, I., Morton, C., Lenoir, G., Swan, D., Tronick, S., Aaronson, S., and Leder, P., 1982, Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells, Proc. Natl. Acad. Sci. USA, 79:7837.PubMedCrossRefGoogle Scholar
  29. Trowbridge, I.S., and Domingo, D.L., 1981, Effect on growth of human tumor cells of anti-transferrin receptor, monoclonal antibody and toxin antibody conjugates, Nature (London), 294:171.CrossRefGoogle Scholar
  30. Trowbridge, I.S., Lesly, J., and Schulte, R., 1982, Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody, J. Cell. Physiol., 112:403.PubMedCrossRefGoogle Scholar
  31. Trowbridge, I.S., and Lopez, F., 1982, Monoclonal antibody to transferrin receptor blocks transferrin binding and inhibits tumor cell growth in vitro, Proc. Natl. Acad. Sci. USA, 79:1175.PubMedCrossRefGoogle Scholar
  32. Trowbridge, I.S., and Omary, M.B., 1981, Human cell surface glycoprotein related to cell proliferation is the receptor for transferrin, Proc. Natl. Acad. Sci. USA, 78:3039.PubMedCrossRefGoogle Scholar
  33. Balmain, A., Ramsden, M., Bowder, G. T., and Smith, J., 1984, Activation of the mouse cellular Harvey-ras gene in chemically induced benign skin papillomas, Nature (London), 307:658.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Alan Diamond
    • 1
  • Joan M. Devine
    • 1
  • Mary-Ann Lane
    • 2
  • Geoffrey M. Cooper
    • 1
  1. 1.Laboratory of Molecular CarcinogenesisHarvard Medical SchoolBostonUSA
  2. 2.Laboratory of Molecular Immunobiology, Dana-Farber Cancer Institute and Dept. of PathologyHarvard Medical SchoolBostonUSA

Personalised recommendations