The Use of Mouse Lymphocyte Clones to Define T-Cell Gene Products and Analyze Lymphocyte Gene Activation
Several systems have facilitated the study of gene activation in eukaryotic cells. Because they have been so well-characterized, mouse T lymphocytes have proven particularly useful for these studies. Many of the molecules expressed on the surface membranes of thymic-dependent lineages have been defined using monoclonal antibodies, biochemical purification, or identification of the genes encoding them.1–5 This differentiation process has been characterized using gene products on cell surfaces to define the cellular stages of T-cell development.6–9 More recently, the molecules that mediate biological functions of T cells have also become established. These include lymphokines, cell surface molecules involved in the recognition of antigen, and cell surface glycoproteins expressed during activation. The technology of T-cell cloning has facilitated the definition of these molecules. T-cell clones promise further to allow insight into the steps that lead to activation. This chapter will review several aspects of mouse T-cell clones. No attempt will be made to review completely the many contributions to this field. Attention will be devoted to the following topics: (1) What T cell clones can be grown in vitro and what are their properties? (2) What essential factors allow continuous propagation of T-cell clones? (3) How can these be used to understand gene activation in eukaryotic cells?
KeywordsConditioned Medium Cell Surface Glycoprotein Mast Cell Growth Factor Clone Mast Cell Human Inducer
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