Gene Transfer as a General Approach to Facilitate the Recombinant DNA Cloning of Surface Antigen Genes
Flow cytometry and the use of monoclonal antibodies have contributed significantly to a number of somatic cell genetic methodologies involving surface antigens, such as gene mapping using somatic cell hybrids, DNA-mediated transformation of recipient cells using cloned surface antigen genes, and, most recently, surface antigen gene cloning. These topics have been previously reviewed.1 In this chapter, I shall discuss recent progress involving the recombinant DNA cloning of surface antigen genes, using the transferrin receptor as a model system.2 The use of eukaryotic cells as host systems for DNA recombinant cloning of non-membrane protein genes has been reviewed recently.3
KeywordsTransferrin Receptor Recipient Cell Primary Transformants Thymidine Kinase Gene Secondary Transformants
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