Enzyme Active Sites
Monoclonal antibodies (MAbs) have provided powerful new tools for analysis of enzyme function. MAbs directed at specific subsites of the enzyme active site allow analysis of catalysis at a new level of resolution even for relatively simple, monomeric enzymes. Because many different MAbs can be prepared to a given enzyme protein or polypeptide complex, and each will be exquisitely specific for a given antigenic determinant or epitope, multiple subunits and associated catalytic functions become potentially separable. In addition, because the binding of MAbs to protein antigens is sensitive to even single residue changes in the antigenic determinant, it is possible to discriminate between very closely related but structurally distinct multiple molecular forms of enzymes.1 This discussion reviews several unique studies in which MAbs have been used to examine catalysis in ways not previously approachable by conventional biochemical methods.
KeywordsInfluenza Virus Sialic Acid Antigenic Determinant Catalytic Center Antigenic Variant
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