Production of Human Monoclonal Antibodies Using a Human-Mouse Fusion Partner
In man, the use of antigen-specific antibodies is an important clinical tool for diagnostic testing (e.g., blood typing for transfusion, tissue typing for transplantation) and for therapy (e.g., prophylaxis of Rh hemolytic disease of the newborn and zoster immune plasma) (Yankee et al., 1969; Grumet et al., 1982; Davey and Zipursky, 1979; Ross, 1962; Zaia et al., 1983). The limited availability or specificity of many of these reagents has placed an important restraint on their use. The production of hybrids between myeloma cell lines and lymphocytes from immunized hosts appears to make possible the unlimited production of monoclonal antibodies to predefined antigens (Köhler and Milstein, 1975). Successful application of this technique, primarily with rodents, is of limited clinical use because xenogeneic immunization with human cells mainly yields antibodies against monomorphic, species-specific antigens rather than polymorphic alloantigens. Moreover, rodent hybridomas have a theoretical limitation for therapeutic use because their secreted antibody would be treated by human recipients as a foreign protein with potential for inducing serum sickness.
KeywordsHerpes Zoster Varicella Zoster Virus Human Monoclonal Antibody Human Lymphoblastoid Cell Line Microtiter Tray
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