Production of Human Monoclonal Antibodies Using Epstein—Barr Virus
The Epstein—Barr virus (EBV) is a human lymphotrophic herpes virus that is the causative agent of infectious mononucleosis (Henle et al., 1968), and is also etiologically associated with two human tumors: African Burkitt lymphoma (Epstein and Achong, 1979) and nasopharyngeal carcinoma (Epstein, 1978). When used in vitro, the virus infects and transforms human B lymphocytes (Pattengale et al., 1973), which will thereafter grow continuously in culture as lymphoblastoid cell lines (Pope et al., 1968). EBV infection also causes polyclonal activation of B lymphocytes, with the synthesis and secretion of immunoglobulin (Rosen et al., 1977). The combination of these two properties of EBV makes it a useful tool for the immortalization of human antibody-secreting cells.
KeywordsHuman Monoclonal Antibody Tetanus Toxoid Infectious Mononucleosis Clone Cell Line Human MABS
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