Factors Generated by Human T-Cell Hybridomas Regulate B-Cell Activation, Polyclonal Differentiation, and Isotype Expression
The interactions between B and T lymphocytes appear to be mediated in large part by factors secreted by activated T cells (Schimpl and Wecker, 1972; Marrack et al., 1982; Yoshizaki et al., 1982). These factors in turn act on various B-cell subpopulations (to induce activation and/or differentiation), other T cells (as a recruitment effort), and monocytes, to effect an immune response. This system of interactions is enormously complex and difficult to characterize, especially since most studies employ either heterogeneous cell populations or cells that secrete multiple factors. This has been a major problem in the study of T-B collaboration. The source of factors is either lectin-or antigen (Ag)-stimulated T cells or T-cell lines, frequently requiring lectin for secretion. Contaminating mitogens can activate the residual T cells in most B-cell preparations and the multiple factors can interact with several different B-cell subpopulations (as well as contaminating T cells), making data interpretation even more complex. Obviously, a monoclonal system as well as constitutive secretion of solitary factors would aid in dissecting specific stages of B-cell activation and differentiation.
KeywordsSoft Agar Common Variable Immunodeficiency Percoll Fraction Reverse Hemolytic Plaque Assay Autologous Mixed Lymphocyte Response
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