Human T-T Hybridomas Specific for Epstein—Barr Virus
Two technologies currently exist to obtain clonal expression of human T cells. One relies on interleukin 2 (IL-2) to expand single-cell cultures. This creates lines of T cells whose maintenance remains totally dependent on the presence of the lymphokine. The second approach, somatic cell hybridization, generates clonal populations of T cells capable of autonomous growth. Successful hybridization is crucially dependent on the phenotype, functional properties, and growth characteristics of the parental cell lines. By appropriate matching of an immortal malignant T-cell line with a normal T cell prior to fusion, a hybrid can be created that expresses a specific molecule or activity in an antigen-specific or -nonspecific manner. The technique has its limitations, but it has resulted in initial successes.
KeywordsHybrid Cell Infectious Mononucleosis Hybrid Clone Inosine Mono Phosphate
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