Human T-T Hybridomas Specific for Epstein—Barr Virus

Generation and Function
  • Mary A. Valentine
  • Dennis A. Carson


Two technologies currently exist to obtain clonal expression of human T cells. One relies on interleukin 2 (IL-2) to expand single-cell cultures. This creates lines of T cells whose maintenance remains totally dependent on the presence of the lymphokine. The second approach, somatic cell hybridization, generates clonal populations of T cells capable of autonomous growth. Successful hybridization is crucially dependent on the phenotype, functional properties, and growth characteristics of the parental cell lines. By appropriate matching of an immortal malignant T-cell line with a normal T cell prior to fusion, a hybrid can be created that expresses a specific molecule or activity in an antigen-specific or -nonspecific manner. The technique has its limitations, but it has resulted in initial successes.


Hybrid Cell Infectious Mononucleosis Hybrid Clone Inosine Mono Phosphate 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Mary A. Valentine
    • 1
  • Dennis A. Carson
    • 1
  1. 1.Scripps Clinic and Research FoundationLa JollaUSA

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