Human T-T Hybridomas Specific for Epstein—Barr Virus

Generation and Function
  • Mary A. Valentine
  • Dennis A. Carson

Abstract

Two technologies currently exist to obtain clonal expression of human T cells. One relies on interleukin 2 (IL-2) to expand single-cell cultures. This creates lines of T cells whose maintenance remains totally dependent on the presence of the lymphokine. The second approach, somatic cell hybridization, generates clonal populations of T cells capable of autonomous growth. Successful hybridization is crucially dependent on the phenotype, functional properties, and growth characteristics of the parental cell lines. By appropriate matching of an immortal malignant T-cell line with a normal T cell prior to fusion, a hybrid can be created that expresses a specific molecule or activity in an antigen-specific or -nonspecific manner. The technique has its limitations, but it has resulted in initial successes.

Keywords

Hybrid Cell Infectious Mononucleosis Hybrid Clone Inosine Mono Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Asada, M., Higuichi, M., Kobayashi, Y. and Osawa, T., 1983, Human T-cell hybridomas producing lymphokines. I. Enchancement of lymphotoxin secretion from human T-cell hybridomas by phorbol myristate acetate, Cell. Immunol. 77: 150–160.PubMedCrossRefGoogle Scholar
  2. Butler, J. L., Muraguchi, A., Lane, H. C., and Fauci, A. S., 1983, Development of a human T-T cell hybridoma secreting B cell growth factor, J. Exp. Med. 157: 60–68.PubMedCrossRefGoogle Scholar
  3. Carson, D. A., Kaye, J., and Seegmiller, J. E., 1978, Differential sensitivity of human leukemic T cell lines and B cell lines to growth inhibition by deoxyadenosine, J. Immunol. 121: 1726–1731PubMedGoogle Scholar
  4. DeFreitas, E. C., Vella, S., Linenbach, A., Zimjewski, C., Koprowski, H., and Croce, C. M., 1982, Antigen-specific human T-cell hybridomas with helper activity, Proc. Natl. Acad. Sci. USA 79: 6646–6650.PubMedCrossRefGoogle Scholar
  5. Epstein, M. A., and Achong, G. B., 1977, Recent progress in Epstein—Barr virus research, Annu. Rev. Microbiol. 31: 421–445.PubMedCrossRefGoogle Scholar
  6. Foung, S. K., Saski, D. T., Grumet, F. C., and Engleman, E. G., 1982, Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine, Proc. Natl. Acad. Sci. USA 79: 7484–7488.PubMedCrossRefGoogle Scholar
  7. Gallagher, G., and Stimson, W. H., 1983, Generation of human T-cell hybrids with the characteristics of human peripheral blood T-lymphocytes, Immunol. Lett. 6: 203–207.PubMedCrossRefGoogle Scholar
  8. Gillis, S., and Watson, J., 1980, Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin-2 producing human leukemia T cell line, J. Exp. Med. 152: 1709–1719.PubMedCrossRefGoogle Scholar
  9. Gillis, S., Ferm, M. M., Ou, W., and Smith, K. A., 1978, T-cell growth factor: Parameters of production and a quantitative microassay for activity, J. Immunol. 120: 2027–2032.PubMedGoogle Scholar
  10. Greene, W. C., Fleisher, T. A., Nelson, D. L., and Waldmann, T. A., 1982, Production of human suppressor T cell hybridomas, J. Immunol. 129: 1986–1991.PubMedGoogle Scholar
  11. Greene, W. C., Depper, J. M., Leonard, W. J., Fleisher, T. A., and Waldmann, T. A., 1983, Construction of human suppressor T cell hybridomas, Lymphokine Res. 2 (2): 53–60.PubMedGoogle Scholar
  12. Grillot-Courvalin, C., Brouet, J., Berger, R., and Bernheim, A., 1981, Establishment of human Tcell hybrid line with suppressive activity, Nature 292: 844–845.PubMedCrossRefGoogle Scholar
  13. Huff, T. F., and Isbizaka, K., 1984, Formation of IgE-binding factors by human T-cell hybridomas, Proc. Natl. Acad. Sci. 81: 1514–1518.PubMedCrossRefGoogle Scholar
  14. Irigoyen, O., Rizzolo, P. V., Thomas, Y., Rogozinski, L., and Chess, L., 1981, Generation of functional human T-cell hybrids, J. Exp. Med. 154: 1827–1837.PubMedCrossRefGoogle Scholar
  15. Kappler, J. W., Skidmore, B., White, J., and Marrack, P., 1981, Antigen-inducible, H-2 restricted, interleukin-2-producing T-cell hybridomas, J. Exp. Med. 153: 1198–1214.PubMedCrossRefGoogle Scholar
  16. Klebe, R. J., and Mancuso, M. G., 1981, Chemicals which promote cell hybridization, Somat. Cell Genet. 7 (4): 473–488.PubMedCrossRefGoogle Scholar
  17. Kobayashi, Y., Asada, M., Higuchi, M., and Osawa, T., 1982, Human T-cell hybridomas producing lymphokines. I. Establishment and characterization of human T-cell hybridomas producing lymphotoxin and migration inhibitory factor, J. Immunol. 128: 2714–2718.PubMedGoogle Scholar
  18. Lakow, E., Tsoukas, C. D., Vaughan, J. H., Altman, A., and Carson, D. A., 1983, Human T-cell hybridomas specific for Epstein—Barr virus-infected B lymphocytes, J. Immunol. 130: 169–172.PubMedGoogle Scholar
  19. Lakow, E. S., Valentine, M. A., Vaughan, J. H., Tsoukas, C. D., and Carson, D. A., 1984, Effects of monoclonal antibodies against lymphocyte surface antigens on interleukin-2 excretion by Epstein—Barr virus-specific human T cell hybridomas, Cell Immunol. 85: 67–74.PubMedCrossRefGoogle Scholar
  20. Le, J., Vilcek, J., Adlik, J. R., Cheung, M. K., Balazs, I., Sarngadharan, M. G., and Prensky, W., 1983, Lymphokine production by human T cell hybridomas, J. Immunol. 130: 1231–1235.PubMedGoogle Scholar
  21. Mayer, L., Fu, S. M., and Kunkel, H. G., 1982, Human T-cell hybridomas secreting factors for IgAspecific help, polyclonal B cell activation, and B cell proliferation, J. Exp. Med. 156: 1860–1865.PubMedCrossRefGoogle Scholar
  22. Murakami, M., and Cathcart, M. K., 1984, Human thymus cell hybridoma secreting a nonspecific immuno-suppressor: participation of T4 positive T lymphocytes and monocytes, Fed. Proc. 43 (6): 1500 (abstract).Google Scholar
  23. Okada, M., Yoshimura, N., Kaieda, T., Yamamura, Y., and Kishimoto, T., 1981, Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules, Proc. Natl. Acad. Sci. USA 78: 7717–7721.PubMedCrossRefGoogle Scholar
  24. Osawa, T., Kobayashi, Y., Asada, M., and Higuchi, M., 1983, Lymphokine-producing human T-cell hybridomas, in: Humoral Factors in Host Defense (Y. Yamamura, ed.), Academic Press, New York, pp. 291–306.Google Scholar
  25. Platsoucas, C. D., Calvelli, T. A., Kunicka, J. E., Lawless, B. D., and Higgins, J. A., 1984, Constitutive production of a suppressor factor by human T-T cell hybridomas, Fed. Proc. 43 (6): 1605 (abstract).Google Scholar
  26. Ruscetti, F. W., and Gallo, R. C., 1981, Human T-lymphocyte growth factor: Regulation of growth and function of T lymphocytes, Blood 57: 379–394.PubMedGoogle Scholar
  27. Schachtschabel, D. O., Lazarus, H., Farber, S., and Foley, G. E., 1966, Sensitivity of cultured human lymphoblasts (CCRF-CEM cells) to inhibition by thymidine, Exp. Cell Res. 43: 512–514.PubMedCrossRefGoogle Scholar
  28. Schwenk, H., and Schneider, U., 1975, Cell cycle dependency of a T-cell marker on lymphoblasts, Blut 31: 299–306.PubMedCrossRefGoogle Scholar
  29. Theodore, A. C., Beer, D. J., Picarella, D. E., Rosenwasser, L. J., and Center, D. M., 1984, Production of lymphotactic lymphokines by human T-T cell hybridomas, Fed. Proc. 43 (6): 1603 (abstract).Google Scholar
  30. Thompson, L. H., and Baker, R., 1973, Isolation of mutants of cultured mammalian cells, in: Methods in Cellular Biology ( D. A. Prescott, ed.), Academic Press, New York, pp. 209–281.Google Scholar
  31. Tsoukas, C. D., Fox, R. I., Slovin, S. F., Carson, D. A., Pelligrino, M., Fong, S., Pasquali, J. L., Ferrone, S., Kung, P., and Vaughan, J. H., 1981, T lymphocyte mediated cytotoxicity against autologous EBV-genome-bearing B cells, J. Immunol. 126: 1742–1746.PubMedGoogle Scholar
  32. Valentine, M. A., Lakow, E. S., Vaughan, J. H., and Carson, D. A., 1983, T cell hybridomas: A new approach for studying the functional properties of T cells in rheumatoid arthritis, Arthritis Rheum. 26: 545 (abstract).Google Scholar
  33. Valentine, M. A., Tsoukas, C. D., Vaughan, J. H., and Carson, D. A., 1984, Human T cell hybrids expressing naturally occurring HLA-DR restricted receptors that recognize EBV-infected B cells, J. Cell. Biochem. (Suppl.) 8A: 275 (abstract).Google Scholar
  34. Vervliet, G., Asma, G., Vossen, J., and Billiau, A., 1983, Isolation and characterization of a human HAT-sensitive T-cell line as a tool for the construction of human T-cell hybridomas, Arch. Int. Physiol. Biochim. 90 (A): B224–225.Google Scholar
  35. Weiser, W., Kawaguchi, T., David, J., and Remold, H., 1984, Generation of human hybridomas producing MIF and of murine hybridomas secreting monoclonal antibodies to these MIF species, Fed. Proc. 43 (6): 1500 (abstract).Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Mary A. Valentine
    • 1
  • Dennis A. Carson
    • 1
  1. 1.Scripps Clinic and Research FoundationLa JollaUSA

Personalised recommendations