Human B-Cell Growth and Differentiation Factors Secreted by T-Cell Hybridomas
Application of the technique of cell hybridization (Köhler and Milstein, 1975) to human and murine T lymphocytes has been an area of recent intense interest and investigation. T-cell hybridomas have been developed that secrete a variety of immunoregulatory factors, such as interleukin 2 (IL-2) (Harwell et al., 1980), killer helper factor (Okada et al.,1981), and factors that help or suppress immunoglobulin (Ig) production (Irigoyen et al., 1981; Grillot-Courvalin and Brouet, 1981). Supernatants from these hybrid cell lines have proven to be a valuable source of monoclonal lymphokines for use in immunochemical as well as functional studies.
KeywordsMixed Lymphocyte Culture Hybrid Cell Line Tritiated Thymidine Incorporation Staphylococcus Aureus Cowan Immunoregulatory Factor
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