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Human B-Cell Growth and Differentiation Factors Secreted by T-Cell Hybridomas

  • Joseph L. Butler
  • Julian L. AmbrusJr.
  • Anthony S. Fauci

Abstract

Application of the technique of cell hybridization (Köhler and Milstein, 1975) to human and murine T lymphocytes has been an area of recent intense interest and investigation. T-cell hybridomas have been developed that secrete a variety of immunoregulatory factors, such as interleukin 2 (IL-2) (Harwell et al., 1980), killer helper factor (Okada et al.,1981), and factors that help or suppress immunoglobulin (Ig) production (Irigoyen et al., 1981; Grillot-Courvalin and Brouet, 1981). Supernatants from these hybrid cell lines have proven to be a valuable source of monoclonal lymphokines for use in immunochemical as well as functional studies.

Keywords

Mixed Lymphocyte Culture Hybrid Cell Line Tritiated Thymidine Incorporation Staphylococcus Aureus Cowan Immunoregulatory Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Butler, J. L.,Muraguchi,A., Lane,H. C., and Fauci, A. S.,1983, Development of a human T-T cell hybridoma secreting B cell growth factor, J. Exp. Med.157:60.Google Scholar
  2. Butler, J. L., Ambrus, J. L., and Fauci, A. S., 1984a, Characterization of monoclonal B cell growth factor (BCGF) produced by a human T-T hybridoma, J. Immunol. 135: 251.Google Scholar
  3. Butler, J. L., Falkoff, R. J. M., and Fauci, A. S., 1984b, Development of a human T cell hybridoma secreting separate B cell growth and differentiation factors, Proc. Natl. Acad. Sci. USA 81: 2475.PubMedCrossRefGoogle Scholar
  4. Falkoff, R. M., Peters, M., and Fauci, A. S., 1982a, T cell enrichment and depletion of human peripheral blood mononuclear cell preparations. Unexpected findings in the study of the functional activities of the separated populations, J. Immunol. Meth. 50: 39.CrossRefGoogle Scholar
  5. Falkoff, R. J. M., Zhu, L. P., and Fauci, A. S., 1982b, Separate signals for human B cell proliferation and differentiation in response to Staphylococcus aureus: Evidence for a two-signal model of B cell activation, J. Immunol. 129: 97.PubMedGoogle Scholar
  6. Foung, S. K. H., Sasaki, D. T., Grumet, F. C., and Engleman, E. G., 1982, Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine, Proc. Natl. Acad. Sci. USA 79: 7484.PubMedCrossRefGoogle Scholar
  7. Grillot-Courvalin, C., and Brouet, J. C., 1981, Establishment of a human T-cell hybrid line with suppressive activity, Nature 292: 844.PubMedCrossRefGoogle Scholar
  8. Harwell, L., Skidmore, B., Marrack, P., and Kappler, J., 1980, Concanavalin A-inducible interleukin-2-producing T cell hybridoma, J. Exp. Med. 152: 893.PubMedCrossRefGoogle Scholar
  9. Haynes, B. F., Hemler, M. E., Mann, D. L., Eisenbarth, G. S., Shelhamer, J., Mostowski, H. S., Thomas, C. A., and Fauci, A. S., 1981, Characterization of a monoclonal antibody (4F2) which binds to human monocytes and to a subset of activated lymphocytes, J. Immunol. 126: 1409.PubMedGoogle Scholar
  10. Irigoyen, O., Rizzolo, P. V., Thomas, Y., Rogozinski, L., and Chess, L., 1981, Generation of functional human T cell hybrids, J. Exp. Med. 154: 1827.PubMedCrossRefGoogle Scholar
  11. Kasahara, T., Oppenheim, J. J., Muraguchi, A., and Fauci, A. S., 1983, Biochemical characterization of human B cell growth factor (BCGF), in: Interleukins, Lymphokines, and Cytokines ( S. Cohen and J. J. Oppenheim, eds.), Academic Press, New York, p. 211.Google Scholar
  12. Kehrl, J. H., Muraguchi, A., and Fauci, A. S., 1984, Human B cell activation and cell cycle progression: Stimulation with anti-p, and Staphylococcus aureus Cowan strain I, Eur. J. Immunol. 14: 115.PubMedCrossRefGoogle Scholar
  13. Kobayashi, Y., Asada, M., Higuchi, M., and Osawa, T., 1982, Human T cell hybridomas producing lymphokines. I. Establishment and characterization of human T cell hybridomas producing lymphotoxin and migration inhibitory factor, J. Immunol. 128: 2714.PubMedGoogle Scholar
  14. Köhler, G., and Milstein, C., 1975, Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256: 495.PubMedCrossRefGoogle Scholar
  15. Maizel, A., Sahasrabuddhe, C., Mehta, S., Morgan, J., Lachman, L., and Ford, R., 1982, Biochemical separation of a human B cell mitogenic factor, Proc. Natl. Acad. Sci. USA 79: 5998.PubMedCrossRefGoogle Scholar
  16. Muraguchi, A., and Fauci, A. S., 1982, Proliferative responses of normal human B lympocytes. Development of an assay system for B cell growth factor (BCGF), J. Immunol. 129: 1104.PubMedGoogle Scholar
  17. Muraguchi, A., Kishimoto, T., Miki, Y., Kuritani, T., Kaieda, T., Yoshizaki, K., and Yamamura, Y., 1981, T cell-replacing factor (TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF, J. Immunol. 127: 412.PubMedGoogle Scholar
  18. Muraguchi, A., Kasahara, T., Oppenheim, J. J., and Fauci, A. S., 1982, B cell growth factor and T cell growth factor produced by mitogen-stimulated normal human peripheral blood T lymphocytes are distinct molecules, J. Immunol. 129: 2486.PubMedGoogle Scholar
  19. Muraguchi, A., Butler, J. L., Kehrl, J. H., and Fauci, A. S., 1983, Differential sensitivity of human B cell subsets to activation signals delivered by anti-p, antibody and proliferative signals delivered by a monoclonal B cell growth factor, J. Exp. Med. 157: 530.CrossRefGoogle Scholar
  20. Okada, M., Yoshimura, N., Kaieda, T., Yamamura, Y., and Kishimoto, T., 1981, Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules, Proc. Natl. Acad. Sci. USA 78: 7717.PubMedCrossRefGoogle Scholar
  21. Okada, M., Sakaguchi, N., Yoshimura, N., Hara, H., Shimizu, K., Yoshida, N., Yoshizaki, K., Kishimoto, S., Yamamura, Y., and Kishimoto, T., 1983, B cell growth factors and B cell differentiation factor from human T hybridomas. Two distinct kinds of B cell growth factor and their synergism in B cell proliferation, J. Exp. Med. 157: 583.PubMedCrossRefGoogle Scholar
  22. Yoshizaki, K., Nakagawa, T., Kaieda, T., Muraguchi, A., Yamamura, Y., and Kishimoto, T., 1982, Induction of proliferation and Ig production in human B leukemic cells by antiimmunoglobulins and T cell factors, J. Immunol. 128: 1296.PubMedGoogle Scholar
  23. Yoshizaki, K., Nakagawa, T., Fukunaga, K., Kaieda, T., Maruyama, S., Kishimoto, S., Yamamura, Y., and Kishimoto, T., 1983, Characterization of human B cell growth factor (BCGF) from cloned T cells or mitogen-stimulated T cells, J. Immunol. 130: 1241.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Joseph L. Butler
    • 1
  • Julian L. AmbrusJr.
    • 1
  • Anthony S. Fauci
    • 1
  1. 1.Laboratory of Immunoregulation, National Institute of Allergy and Infectious DiseaseNational Institutes of HealthBethesdaUSA

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