Principles of in Vitro Immunization of Human B Lymphocytes
The development by Mishell and Dutton of a culture technique for immunization of mouse spleen cells (Mishell and Dutton, 1967) was an important advance in the study of the humoral immune response since it provided a convenient and versatile way to study cellular and subcellular events in the response.
KeywordsSuppressor Cell Mouse Spleen Cell Human Lymphoid Cell Acute Monocytic Leukemia OKT8 Antibody
Unable to display preview. Download preview PDF.
- Cavagnaro, J., and Osband, M., 1983, Successful in vitro primary immunization of human peripheral blood mononuclear cells and its role in the development of human-derived monoclonal antibodies, Bio Techniques 1: 30–36.Google Scholar
- Hoffmann, M. K., 1981, B cell activation in the humoral immune response to sheep erythrocytes. Role of helper factors produced by macrophages and T cells, in: B Lymphocytes in the Immune Response ( N. Klinman, D. E. Mosier, I. Sher, and E. S. Vitetta, eds.), Elsevier/North-Holland, New York, pp. 339–344.Google Scholar
- Rheinherz, E. L., Kung, P. C., Goldstein, G., and Schlossman, S. F., 1979, Further characterization of the human inducer T cell subset by monoclonal antibody, J. Immunol. 123: 2894–2896.Google Scholar
- Rheinherz, E. L., Kung, P. C., Goldstein, G., and Schlossman, S. F., 1980, A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset defined by a heteroantiserum termed TH2, J. Immunol. 124: 1301–1307.Google Scholar
- Schimpl, A., Hübner, A., Wong, L., and Wecker, E., 1980, Characteristics of late acting T cell replacing factor, Behring Inst. Mitt. 67: 221–229.Google Scholar
- Schurman, R. K. B., Gelfand, E. B., and Dosh, H. M., 1980, Polyclonal activation of human lymphocytes in vitro. I. Characterization of the lymphocyte response to a T cell-independent B cell mitogen, J. Immunol. 125: 820–826.Google Scholar