Principles of in Vitro Immunization of Human B Lymphocytes

  • Michael K. Hoffmann
  • John A. Hirst


The development by Mishell and Dutton of a culture technique for immunization of mouse spleen cells (Mishell and Dutton, 1967) was an important advance in the study of the humoral immune response since it provided a convenient and versatile way to study cellular and subcellular events in the response.


Suppressor Cell Mouse Spleen Cell Human Lymphoid Cell Acute Monocytic Leukemia OKT8 Antibody 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Michael K. Hoffmann
    • 1
  • John A. Hirst
    • 1
  1. 1.Memorial Sloan-Kettering Cancer CenterNew YorkUSA

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