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Human Monoclonal Autoantibodies Reactive with Multiple Organs

  • Floyd Taub
  • Jo Satoh
  • Carlo Garzelli
  • Karim Essani
  • Abner Louis Notkins

Abstract

Human monoclonal antibodies that react with normal tissues (autoantibodies) have recently been prepared from peripheral blood lymphocytes (PBLs) of patients with insulin-dependent diabetes mellitus (IDDM) and thyroiditis. This chapter describes the methods by which these monoclonal autoantibodies have been obtained and evaluates their reactivity with normal tissue antigens. In addition, the capacity of these monoclonal autoantibodies to react with antigens in multiple organs is described.

Keywords

Cloning Efficiency Stratify Squamous Epithelium Human Myeloma Cell Nylon Wool Column Monoclonal Autoantibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bird, G., Britton, S., Ernberg, I., and Nilsson, K., 1981, Characteristics of Epstein—Barr virus activation of human B lymphocytes, J. Exp. Med. 154: 832–839.PubMedCrossRefGoogle Scholar
  2. Garzelli, C., Taub, F. E., Scharff, J. E., Prabhakar, B. S., Ginsberg-Fellner, F., and Notkins, A. L., 1984, Epstein—Barr virus transformed lymphocytes produce monoclonal autoantibodies that react with antigens in multiple organs, J. Virol. 52: 722–725.PubMedGoogle Scholar
  3. Haspel, M. V., Onodera, T., Prabhakar, B. S., McClintock, P. R., Essani, K., Ray, U. R., Yagihashi, S., and Notkins, A. L., 1983, Multiple organ-reactive monoclonal autoantibodies, Nature 304: 73–76.PubMedCrossRefGoogle Scholar
  4. Hsu, S. M., Raine, L., and Fanger, H., 1981, The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures, J. Histochem. Cytochem. 29: 577–580.PubMedCrossRefGoogle Scholar
  5. Kearney, J. F., 1984, Hybridomas and monoclonal antibodies, in: Fundamental Immunology (W. E. Paul, ed.), Raven Press, New York, pp. 751–766.Google Scholar
  6. Lane, H. C., Shelhamer, J. H., Mostowski, H. S., and Fauci, A. S., 1982, Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual, J. Exp. Med. 155: 333–338.PubMedCrossRefGoogle Scholar
  7. McKearn, T. J., 1980, Cloning of hybridoma cells by limiting dilution in fluid phase, in: Monoclonal Antibodies ( R. H. Kennett, T. J. McKearn, and K. B. Bechtol, eds.), Plenum Press, New York, p. 374.Google Scholar
  8. Miller, G., and Lipman, M., 1973, Release of infectious Epstein-Barr virus by transformed marmoset leukocytes, Proc. Natl. Acad. Sci. USA 70: 90–194.CrossRefGoogle Scholar
  9. Prabhakar, B. S., Saegusa, J., Onodera, T., and Notkins, A. L., 1984, Lymphocytes capable of making monoclonal autoantibodies that react with multiple organs are a common feature of the normal B cell repertoire, J. Immunol. 133: 2815–2817.PubMedGoogle Scholar
  10. Rubin, R. L., Balderas, R. S., Tan, E. M., Dixon, F. J., and Theofilopoulos, A. N., 1984, Multiple autoantigen binding capabilities of mouse monoclonal antibodies selected for rheumatoid factor activity, J. Exp. Med. 159: 1429–1440.PubMedCrossRefGoogle Scholar
  11. Satoh, J., Prabhakar, B. S., Haspel, M. V., Ginsberg-Fellner, F., and Notkins, A. L., 1983, Human monoclonal autoantibodies that react with multiple endocrine organs, N. Engl. J. Med. 309: 217–220.PubMedCrossRefGoogle Scholar
  12. Satoh, J., Essani, K., McClintock, P. R., and Notkins, A. L., 1984, Human multiple organ-reactive monoclonal autoantibody recognizes growth hormone and a 35K protein, J. Clin. Invest. 74: 1526–1531.PubMedCrossRefGoogle Scholar
  13. Shoenfeld, Y., Rauch, J., Massicotte, H., Datta, S. K., André-Schwartz, J., Stollar, B. D., and Schwartz, R. S., 1983, Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas, N. Engl. J. Med. 308: 414–420.PubMedCrossRefGoogle Scholar
  14. Takasugi, M., Mickey, M. R., and Levine, P. H., 1982, Natural and antibody-dependent cell-mediated cytotoxicity to cultured target cells superinfected with Epstein—Barr virus, Cancer Res. 42: 1208–1214.PubMedGoogle Scholar
  15. Trizio, D., and Cudkowicz, G., 1974, Separation of T and B lymphocytes by nylon wool columns: Evaluation of efficacy by functional assay in vivo, J. Immunol. 113: 1093–1097.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Floyd Taub
    • 1
  • Jo Satoh
    • 1
  • Carlo Garzelli
    • 1
  • Karim Essani
    • 1
  • Abner Louis Notkins
    • 1
  1. 1.Laboratory of Oral Medicine, National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA

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